Abstract
The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5234-5236 |
| Number of pages | 3 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 90 |
| Issue number | 11 |
| State | Published - Jun 1 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Transfection of the malaria parasite and expression of firefly luciferase. / Goonewardene, Renu; Daily, Johanna P.; Kaslow, David; Sullivan, Terrence J.; Duffy, Patrick; Carter, Richard; Mendis, Kamini; Wirth, Dyann.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 11, 01.06.1993, p. 5234-5236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transfection of the malaria parasite and expression of firefly luciferase
AU - Goonewardene, Renu
AU - Daily, Johanna P.
AU - Kaslow, David
AU - Sullivan, Terrence J.
AU - Duffy, Patrick
AU - Carter, Richard
AU - Mendis, Kamini
AU - Wirth, Dyann
PY - 1993/6/1
Y1 - 1993/6/1
N2 - The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.
AB - The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.
UR - http://www.scopus.com/inward/record.url?scp=0027160557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027160557&partnerID=8YFLogxK
M3 - Article
C2 - 8506371
AN - SCOPUS:0027160557
VL - 90
SP - 5234
EP - 5236
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 11
ER -