Transfection of the malaria parasite and expression of firefly luciferase

Renu Goonewardene, Johanna Daily, David Kaslow, Terrence J. Sullivan, Patrick Duffy, Richard Carter, Kamini Mendis, Dyann Wirth

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.

Original languageEnglish (US)
Pages (from-to)5234-5236
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number11
DOIs
StatePublished - Jun 1 1993
Externally publishedYes

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