Transfection of the malaria parasite and expression of firefly luciferase

Renu Goonewardene; Johanna Daily; David Kaslow; Terrence J. Sullivan; Patrick Duffy; Richard Carter; Kamini Mendis; Dyann Wirth

doi:10.1073/pnas.90.11.5234

Transfection of the malaria parasite and expression of firefly luciferase

Renu Goonewardene, Johanna Daily, David Kaslow, Terrence J. Sullivan, Patrick Duffy, Richard Carter, Kamini Mendis, Dyann Wirth

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.

Original language	English (US)
Pages (from-to)	5234-5236
Number of pages	3
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	11
DOIs	https://doi.org/10.1073/pnas.90.11.5234
State	Published - Jun 1 1993
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.90.11.5234

Fingerprint Dive into the research topics of 'Transfection of the malaria parasite and expression of firefly luciferase'. Together they form a unique fingerprint.

Cite this

Transfection of the malaria parasite and expression of firefly luciferase. / Goonewardene, Renu; Daily, Johanna; Kaslow, David; Sullivan, Terrence J.; Duffy, Patrick; Carter, Richard; Mendis, Kamini; Wirth, Dyann.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 11, 01.06.1993, p. 5234-5236.

Research output: Contribution to journal › Article › peer-review

@article{f5f8c7c53c0a4d489b42eae34163f989,

title = "Transfection of the malaria parasite and expression of firefly luciferase",

abstract = "The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.",

author = "Renu Goonewardene and Johanna Daily and David Kaslow and Sullivan, {Terrence J.} and Patrick Duffy and Richard Carter and Kamini Mendis and Dyann Wirth",

year = "1993",

month = jun,

day = "1",

doi = "10.1073/pnas.90.11.5234",

language = "English (US)",

volume = "90",

pages = "5234--5236",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "11",

}

TY - JOUR

T1 - Transfection of the malaria parasite and expression of firefly luciferase

AU - Goonewardene, Renu

AU - Daily, Johanna

AU - Kaslow, David

AU - Sullivan, Terrence J.

AU - Duffy, Patrick

AU - Carter, Richard

AU - Mendis, Kamini

AU - Wirth, Dyann

PY - 1993/6/1

Y1 - 1993/6/1

N2 - The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.

AB - The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.

UR - http://www.scopus.com/inward/record.url?scp=0027160557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027160557&partnerID=8YFLogxK

U2 - 10.1073/pnas.90.11.5234

DO - 10.1073/pnas.90.11.5234

M3 - Article

C2 - 8506371

AN - SCOPUS:0027160557

VL - 90

SP - 5234

EP - 5236

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11

ER -

Transfection of the malaria parasite and expression of firefly luciferase

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this