Transfection of the malaria parasite and expression of firefly luciferase

Renu Goonewardene; Johanna Daily; David Kaslow; Terrence J. Sullivan; Patrick Duffy; Richard Carter; Kamini Mendis; Dyann Wirth

doi:10.1073/pnas.90.11.5234

Transfection of the malaria parasite and expression of firefly luciferase

Renu Goonewardene, Johanna Daily, David Kaslow, Terrence J. Sullivan, Patrick Duffy, Richard Carter, Kamini Mendis, Dyann Wirth

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102 Scopus citations

Abstract

The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.

Original language	English (US)
Pages (from-to)	5234-5236
Number of pages	3
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	11
DOIs	https://doi.org/10.1073/pnas.90.11.5234
State	Published - Jun 1 1993
Externally published	Yes

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abstract = "The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.",

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T1 - Transfection of the malaria parasite and expression of firefly luciferase

AU - Goonewardene, Renu

AU - Daily, Johanna

AU - Kaslow, David

AU - Sullivan, Terrence J.

AU - Duffy, Patrick

AU - Carter, Richard

AU - Mendis, Kamini

AU - Wirth, Dyann

PY - 1993/6/1

Y1 - 1993/6/1

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AB - The goal of this work is to develop a method for the functional analysis of malaria genes using the method of DNA transfection. We have developed a transient transfection vector by constructing a chinteric gene in which the firefly luciferase gene was inserted in frame into the coding region of the pgs28 gene of Plasmodium gallinaceum. This plasmid DNA was introduced into P. gallinaceum gametes and fertilized zygotes by electroporation, and luciferase expression was assayed after 24 hr. This report of successful introduction and expression of a foreign gene in a malaria parasite demonstrates the feasibility of this approach to developing methods for the functional analysis of parasite genes.

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Transfection of the malaria parasite and expression of firefly luciferase

Abstract

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