Abstract
Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 623-627 |
| Number of pages | 5 |
| Journal | Leukemia Research |
| Volume | 14 |
| Issue number | 7 |
| DOIs | |
| State | Published - 1990 |
Keywords
- Glucocorticoid
- T-lymphocyte
- cytotoxicity
- dexamethasone
- neomycin phosphotransferase
- transfection
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research
Fingerprint Dive into the research topics of 'Transfection of a T-cell line with neo increases dexamethasone cytotoxicity'. Together they form a unique fingerprint.
Cite this
De Cuevillas, F., & Lachman, H. (1990). Transfection of a T-cell line with neo increases dexamethasone cytotoxicity. Leukemia Research, 14(7), 623-627. https://doi.org/10.1016/0145-2126(90)90017-4