Abstract
Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance.
Original language | English (US) |
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Pages (from-to) | 623-627 |
Number of pages | 5 |
Journal | Leukemia Research |
Volume | 14 |
Issue number | 7 |
DOIs | |
State | Published - 1990 |
Keywords
- Glucocorticoid
- T-lymphocyte
- cytotoxicity
- dexamethasone
- neomycin phosphotransferase
- transfection
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research