Abstract
Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 623-627 |
| Number of pages | 5 |
| Journal | Leukemia Research |
| Volume | 14 |
| Issue number | 7 |
| DOIs | |
| State | Published - 1990 |
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Keywords
- cytotoxicity
- dexamethasone
- Glucocorticoid
- neomycin phosphotransferase
- T-lymphocyte
- transfection
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology
Cite this
Transfection of a T-cell line with neo increases dexamethasone cytotoxicity. / De Cuevillas, Fernando; Lachman, Herbert M.
In: Leukemia Research, Vol. 14, No. 7, 1990, p. 623-627.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transfection of a T-cell line with neo increases dexamethasone cytotoxicity
AU - De Cuevillas, Fernando
AU - Lachman, Herbert M.
PY - 1990
Y1 - 1990
N2 - Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance.
AB - Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance.
KW - cytotoxicity
KW - dexamethasone
KW - Glucocorticoid
KW - neomycin phosphotransferase
KW - T-lymphocyte
KW - transfection
UR - http://www.scopus.com/inward/record.url?scp=0025366066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025366066&partnerID=8YFLogxK
U2 - 10.1016/0145-2126(90)90017-4
DO - 10.1016/0145-2126(90)90017-4
M3 - Article
C2 - 2167404
AN - SCOPUS:0025366066
VL - 14
SP - 623
EP - 627
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 7
ER -