Transfection of a T-cell line with neo increases dexamethasone cytotoxicity

Fernando De Cuevillas, Herbert Lachman

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance.

Original languageEnglish (US)
Pages (from-to)623-627
Number of pages5
JournalLeukemia Research
Volume14
Issue number7
DOIs
StatePublished - 1990

Keywords

  • Glucocorticoid
  • T-lymphocyte
  • cytotoxicity
  • dexamethasone
  • neomycin phosphotransferase
  • transfection

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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