Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi

Daniel Adesse, Luciana Ribeiro Garzoni, Maria De Nazareth Meirelles, Dumitru A. Iacobas, Sanda Iacobas, David C. Spray, Herbert B. Tanowitz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L6E9 myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.

Original languageEnglish (US)
Pages (from-to)846-854
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume82
Issue number5
DOIs
StatePublished - May 2010

Fingerprint

Myoblasts
Trypanosoma cruzi
Cell Line
Genes
Infection
Oligonucleotide Array Sequence Analysis
Transcriptome
Brazil
Parasites
RNA
Gene Expression

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi. / Adesse, Daniel; Garzoni, Luciana Ribeiro; Meirelles, Maria De Nazareth; Iacobas, Dumitru A.; Iacobas, Sanda; Spray, David C.; Tanowitz, Herbert B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 82, No. 5, 05.2010, p. 846-854.

Research output: Contribution to journalArticle

Adesse, Daniel ; Garzoni, Luciana Ribeiro ; Meirelles, Maria De Nazareth ; Iacobas, Dumitru A. ; Iacobas, Sanda ; Spray, David C. ; Tanowitz, Herbert B. / Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi. In: American Journal of Tropical Medicine and Hygiene. 2010 ; Vol. 82, No. 5. pp. 846-854.
@article{49160d94a0794b968266823197e71de4,
title = "Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi",
abstract = "We examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L6E9 myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17{\%} significantly altered genes), whereas the Y strain altered only 6{\%} of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.",
author = "Daniel Adesse and Garzoni, {Luciana Ribeiro} and Meirelles, {Maria De Nazareth} and Iacobas, {Dumitru A.} and Sanda Iacobas and Spray, {David C.} and Tanowitz, {Herbert B.}",
year = "2010",
month = "5",
doi = "10.4269/ajtmh.2010.09-0399",
language = "English (US)",
volume = "82",
pages = "846--854",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "5",

}

TY - JOUR

T1 - Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi

AU - Adesse, Daniel

AU - Garzoni, Luciana Ribeiro

AU - Meirelles, Maria De Nazareth

AU - Iacobas, Dumitru A.

AU - Iacobas, Sanda

AU - Spray, David C.

AU - Tanowitz, Herbert B.

PY - 2010/5

Y1 - 2010/5

N2 - We examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L6E9 myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.

AB - We examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L6E9 myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.

UR - http://www.scopus.com/inward/record.url?scp=77952737414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952737414&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.2010.09-0399

DO - 10.4269/ajtmh.2010.09-0399

M3 - Article

C2 - 20439965

AN - SCOPUS:77952737414

VL - 82

SP - 846

EP - 854

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 5

ER -