Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi

Daniel Adesse, Luciana Ribeiro Garzoni, Maria De Nazareth Meirelles, Dumitru A. Iacobas, Sanda Iacobas, David C. Spray, Herbert B. Tanowitz

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L6E9 myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.

Original languageEnglish (US)
Pages (from-to)846-854
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume82
Issue number5
DOIs
StatePublished - May 2010

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi'. Together they form a unique fingerprint.

  • Cite this