Transcriptomic characterization of four classes of cell-cell/cell-matrix genes in brains and hearts of wild type and connexin43 null mice.

D. A. Iacobaş, Marcia Urban, Sanda Iacobaş, David C. Spray

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have used a highly quantifiable cDNA microarray method to determine the stabilities and expression levels within gene families involved in cell-cell and cell-matrix interactions in neonatal mouse brain and heart. In addition, we have characterized the extent to which deletion of the gap junction protein connexin43 (Cx43) affects these characteristics. Our observations for individual genes revealed a range of differences and variabilities in transcription level among family members; calculation of the genomic patholog (a global measure of gene expression alteration) indicates that these cell interaction genes contribute disproportionately to the overall phenotype. We found significant transcriptomic differences between brain and heart, that deletion of Cx43 considerably decreased gene expression variability and that the average contribution to the pathology of the genes whose encoded proteins are involved in cell-cell or cell-matrix interaction in the Cx43-null mice was about twenty times higher than that of other genes. These findings indicate that gap junction gene expression influences the expression of other genes involved in intercellular and cell-substrate interaction and that expression of these genes is under strong regulatory pressure in the Cx43-null mouse, presumably representing a compensatory response to Cx43 deletion.

Original languageEnglish (US)
Pages (from-to)91-116
Number of pages26
JournalRomanian journal of physiology : physiological sciences / [Academia de Stiinte Medicale]
Volume39-40
StatePublished - 2002

Fingerprint

Connexin 43
Cell Communication
Gene Expression
Brain
Genes
Connexins
Gap Junctions
Oligonucleotide Array Sequence Analysis
Pathology
Phenotype
Pressure
Proteins

Cite this

@article{d876b7473da9461eb0dd07452447f017,
title = "Transcriptomic characterization of four classes of cell-cell/cell-matrix genes in brains and hearts of wild type and connexin43 null mice.",
abstract = "We have used a highly quantifiable cDNA microarray method to determine the stabilities and expression levels within gene families involved in cell-cell and cell-matrix interactions in neonatal mouse brain and heart. In addition, we have characterized the extent to which deletion of the gap junction protein connexin43 (Cx43) affects these characteristics. Our observations for individual genes revealed a range of differences and variabilities in transcription level among family members; calculation of the genomic patholog (a global measure of gene expression alteration) indicates that these cell interaction genes contribute disproportionately to the overall phenotype. We found significant transcriptomic differences between brain and heart, that deletion of Cx43 considerably decreased gene expression variability and that the average contribution to the pathology of the genes whose encoded proteins are involved in cell-cell or cell-matrix interaction in the Cx43-null mice was about twenty times higher than that of other genes. These findings indicate that gap junction gene expression influences the expression of other genes involved in intercellular and cell-substrate interaction and that expression of these genes is under strong regulatory pressure in the Cx43-null mouse, presumably representing a compensatory response to Cx43 deletion.",
author = "Iacobaş, {D. A.} and Marcia Urban and Sanda Iacobaş and Spray, {David C.}",
year = "2002",
language = "English (US)",
volume = "39-40",
pages = "91--116",
journal = "Romanian journal of physiology : physiological sciences / [Academia de Stiinte Medicale]",
issn = "1223-4974",
publisher = "Editions de l'Academie Republique Populaire",

}

TY - JOUR

T1 - Transcriptomic characterization of four classes of cell-cell/cell-matrix genes in brains and hearts of wild type and connexin43 null mice.

AU - Iacobaş, D. A.

AU - Urban, Marcia

AU - Iacobaş, Sanda

AU - Spray, David C.

PY - 2002

Y1 - 2002

N2 - We have used a highly quantifiable cDNA microarray method to determine the stabilities and expression levels within gene families involved in cell-cell and cell-matrix interactions in neonatal mouse brain and heart. In addition, we have characterized the extent to which deletion of the gap junction protein connexin43 (Cx43) affects these characteristics. Our observations for individual genes revealed a range of differences and variabilities in transcription level among family members; calculation of the genomic patholog (a global measure of gene expression alteration) indicates that these cell interaction genes contribute disproportionately to the overall phenotype. We found significant transcriptomic differences between brain and heart, that deletion of Cx43 considerably decreased gene expression variability and that the average contribution to the pathology of the genes whose encoded proteins are involved in cell-cell or cell-matrix interaction in the Cx43-null mice was about twenty times higher than that of other genes. These findings indicate that gap junction gene expression influences the expression of other genes involved in intercellular and cell-substrate interaction and that expression of these genes is under strong regulatory pressure in the Cx43-null mouse, presumably representing a compensatory response to Cx43 deletion.

AB - We have used a highly quantifiable cDNA microarray method to determine the stabilities and expression levels within gene families involved in cell-cell and cell-matrix interactions in neonatal mouse brain and heart. In addition, we have characterized the extent to which deletion of the gap junction protein connexin43 (Cx43) affects these characteristics. Our observations for individual genes revealed a range of differences and variabilities in transcription level among family members; calculation of the genomic patholog (a global measure of gene expression alteration) indicates that these cell interaction genes contribute disproportionately to the overall phenotype. We found significant transcriptomic differences between brain and heart, that deletion of Cx43 considerably decreased gene expression variability and that the average contribution to the pathology of the genes whose encoded proteins are involved in cell-cell or cell-matrix interaction in the Cx43-null mice was about twenty times higher than that of other genes. These findings indicate that gap junction gene expression influences the expression of other genes involved in intercellular and cell-substrate interaction and that expression of these genes is under strong regulatory pressure in the Cx43-null mouse, presumably representing a compensatory response to Cx43 deletion.

UR - http://www.scopus.com/inward/record.url?scp=21444439135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21444439135&partnerID=8YFLogxK

M3 - Article

C2 - 15984671

AN - SCOPUS:21444439135

VL - 39-40

SP - 91

EP - 116

JO - Romanian journal of physiology : physiological sciences / [Academia de Stiinte Medicale]

JF - Romanian journal of physiology : physiological sciences / [Academia de Stiinte Medicale]

SN - 1223-4974

ER -