Transcriptomic and metabonomic profiling reveal synergistic effects of quercetin and resveratrol supplementation in high fat diet fed mice

Mingmei Zhou, Shidong Wang, Aihua Zhao, Ke Wang, Ziquan Fan, Hongzhou Yang, Wen Liao, Si Bao, Linjing Zhao, Yinan Zhang, Yongqing Yang, Yunping Qiu, Guoxiang Xie, Houkai Li, Wei Jia

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Dietary quercetin and resveratrol have been frequently used in treating various diseases, but the underlying mechanisms are not entirely clear. Here, we report combined transcriptomic and metabonomic profiling that showed that the combined supplementation with quercetin and resveratrol produced synergistic effects on a high-fat diet-induced metabolic phenotype in mice. Histological and phenotypic improvements in serum and hepatic total cholesterol, insulin, fasting blood glucose, and HbA1c were also observed in mice receiving combined quercetin and resveratrol supplementation. This combined quercetin and resveratrol supplementation resulted in significant restoration of gene sets in functional pathways of glucose/lipid metabolism, liver function, cardiovascular system, and inflammation/immunity, which were altered by high fat diet feeding. The integration of transcriptomic and metabonomic data indicated quercetin and resveratrol supplementation enhanced processes of glycolysis and fatty acid oxidation, as well as suppressed gluconeogenesis. These alterations discovered at both the transcriptional and metabolic levels highlight the significance of combined "omics" platforms for elucidating mechanistic pathways altered by dietary polyphenols, such as quercetin and resveratrol, in a synergistic manner.

Original languageEnglish (US)
Pages (from-to)4961-4971
Number of pages11
JournalJournal of Proteome Research
Volume11
Issue number10
DOIs
StatePublished - Oct 5 2012
Externally publishedYes

Fingerprint

Metabolomics
Quercetin
High Fat Diet
Nutrition
Fats
Cardiovascular system
Gluconeogenesis
Liver
Polyphenols
Glycolysis
Cardiovascular System
Lipid Metabolism
Restoration
Blood Glucose
resveratrol
Immunity
Fasting
Fatty Acids
Genes
Cholesterol

Keywords

  • fatty liver
  • GC/MS
  • high fat diet
  • metabonomics
  • quercetin
  • resveratrol
  • transcriptomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Transcriptomic and metabonomic profiling reveal synergistic effects of quercetin and resveratrol supplementation in high fat diet fed mice. / Zhou, Mingmei; Wang, Shidong; Zhao, Aihua; Wang, Ke; Fan, Ziquan; Yang, Hongzhou; Liao, Wen; Bao, Si; Zhao, Linjing; Zhang, Yinan; Yang, Yongqing; Qiu, Yunping; Xie, Guoxiang; Li, Houkai; Jia, Wei.

In: Journal of Proteome Research, Vol. 11, No. 10, 05.10.2012, p. 4961-4971.

Research output: Contribution to journalArticle

Zhou, M, Wang, S, Zhao, A, Wang, K, Fan, Z, Yang, H, Liao, W, Bao, S, Zhao, L, Zhang, Y, Yang, Y, Qiu, Y, Xie, G, Li, H & Jia, W 2012, 'Transcriptomic and metabonomic profiling reveal synergistic effects of quercetin and resveratrol supplementation in high fat diet fed mice', Journal of Proteome Research, vol. 11, no. 10, pp. 4961-4971. https://doi.org/10.1021/pr3004826
Zhou, Mingmei ; Wang, Shidong ; Zhao, Aihua ; Wang, Ke ; Fan, Ziquan ; Yang, Hongzhou ; Liao, Wen ; Bao, Si ; Zhao, Linjing ; Zhang, Yinan ; Yang, Yongqing ; Qiu, Yunping ; Xie, Guoxiang ; Li, Houkai ; Jia, Wei. / Transcriptomic and metabonomic profiling reveal synergistic effects of quercetin and resveratrol supplementation in high fat diet fed mice. In: Journal of Proteome Research. 2012 ; Vol. 11, No. 10. pp. 4961-4971.
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