Transcriptomic alterations in Trypanosoma cruzi-infected cardiac myocytes

Regina Coeli dos Santos Goldenberg, Dumitru A. Iacobas, Sanda Iacobas, Leonardo Lima Rocha, Fabio da Silva de Azevedo Fortes, Leandro Vairo, Fnu Nagajyothi, Antonio Carlos Campos de Carvalho, Herbert B. Tanowitz, David C. Spray

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20 Scopus citations

Abstract

Trypanosoma cruzi infection is a major cause of cardiomyopathy. Previous gene profiling studies of infected mouse hearts have revealed prominent changes in gene expression within many functional pathways. This variety of transcriptomic changes in infected mice raises the question of whether gene expression alterations in whole hearts are due to changes in infected cardiac myocytes or other cells or even to systemic effects of the infection on the heart. We employed microarrays to examine infected cardiac myocyte cultures 48 h post-infection. Statistical comparison of gene expression levels of 7624 well annotated unigenes in four independent cultures of infected and uninfected myocytes detected substantial (≥1.5 absolute fold changes) in 420 (5.5%) of the sampled genes. Major categories of affected genes included those involved in immune response, extracellular matrix and cell adhesion. These findings on infected cardiac myocytes in culture reveal that alterations in cardiac gene expression described in Chagas disease are the consequence of both direct infection of the myocytes themselves as well as resulting from the presence of other cell types in the myocardium and systemic effects of infection.

Original languageEnglish (US)
Pages (from-to)1140-1149
Number of pages10
JournalMicrobes and Infection
Volume11
Issue number14-15
DOIs
StatePublished - Dec 1 2009

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Keywords

  • Cardiac myocyte culture
  • Cardiomyopathy
  • Chagas disease
  • Microarray
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Goldenberg, R. C. D. S., Iacobas, D. A., Iacobas, S., Rocha, L. L., da Silva de Azevedo Fortes, F., Vairo, L., Nagajyothi, F., Campos de Carvalho, A. C., Tanowitz, H. B., & Spray, D. C. (2009). Transcriptomic alterations in Trypanosoma cruzi-infected cardiac myocytes. Microbes and Infection, 11(14-15), 1140-1149. https://doi.org/10.1016/j.micinf.2009.08.009