Transcriptional activation of placental growth factor by the forkhead/winged helix transcription factor FoxD1

Hong Zhang, Rachel Palmer, Xiaobo Gao, Jordan Kreidberg, William Gerald, Lili Hsiao, Roderick V. Jensen, Steven R. Gullans, Daniel A. Haber

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Stromal-epithelial interactions play an important role in renal organogenesis [1]. Expression of the forkhead/winged helix transcription factor FoxD1 (BF-2) is restricted to stromal cells in the embryonic renal cortex, but it mediates its effects on the adjacent ureteric bud and metanephric mesenchyme, which fail to grow and differentiate in BF-2 null mice [2]. BF-2 is therefore likely to regulate transcription of factors secreted by stromal cells that modulate the differentiation of neighboring epithelial cells. Here, we used cells with inducible expression of BF-2, combined with microarray analysis, to identify Placental Growth Factor (PIGF), a Vascular Endothelial Growth Factor (VEGF) family member previously implicated in angiogenesis, as a downstream target of BF-2. BF-2 binds to a conserved HNF3β site in the PIGF promoter and activates transcription. PIGF is precisely coexpressed with BF-2, both temporally and spatially, within the developing renal stroma, and it is completely absent in BF-2 null kidney stroma. Addition of PIGF to in vitro kidney organ cultures stimulates branching of the ureteric bud. Our observations indicate that PIGF is a direct and physiologically relevant transcriptional target of BF-2. The contribution of PIGF toward stromal signals that regulate epithelial differentiation suggests novel functions for a growth factor previously implicated in reactive angiogenesis.

Original languageEnglish (US)
Pages (from-to)1625-1629
Number of pages5
JournalCurrent Biology
Volume13
Issue number18
DOIs
StatePublished - Sep 16 2003
Externally publishedYes

Fingerprint

Winged-Helix Transcription Factors
transcriptional activation
growth factors
Transcriptional Activation
Intercellular Signaling Peptides and Proteins
transcription factors
Chemical activation
kidneys
Kidney
Transcription
Microarrays
Vascular Endothelial Growth Factor A
stromal cells
Stromal Cells
angiogenesis
Transcription Factors
buds
organ culture
Organogenesis
vascular endothelial growth factors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Transcriptional activation of placental growth factor by the forkhead/winged helix transcription factor FoxD1. / Zhang, Hong; Palmer, Rachel; Gao, Xiaobo; Kreidberg, Jordan; Gerald, William; Hsiao, Lili; Jensen, Roderick V.; Gullans, Steven R.; Haber, Daniel A.

In: Current Biology, Vol. 13, No. 18, 16.09.2003, p. 1625-1629.

Research output: Contribution to journalArticle

Zhang, H, Palmer, R, Gao, X, Kreidberg, J, Gerald, W, Hsiao, L, Jensen, RV, Gullans, SR & Haber, DA 2003, 'Transcriptional activation of placental growth factor by the forkhead/winged helix transcription factor FoxD1', Current Biology, vol. 13, no. 18, pp. 1625-1629. https://doi.org/10.1016/j.cub.2003.08.054
Zhang, Hong ; Palmer, Rachel ; Gao, Xiaobo ; Kreidberg, Jordan ; Gerald, William ; Hsiao, Lili ; Jensen, Roderick V. ; Gullans, Steven R. ; Haber, Daniel A. / Transcriptional activation of placental growth factor by the forkhead/winged helix transcription factor FoxD1. In: Current Biology. 2003 ; Vol. 13, No. 18. pp. 1625-1629.
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