Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein

D. Smotkin, F. O. Wettstein

Research output: Contribution to journalArticle

483 Scopus citations

Abstract

Human papillomavirus type 16 DNA and RNA were characterized in the cervical cancer-derived CaSki cell line, which contains only integrated DNA, and in a cervical cancer, which contains predominantly plasmid DNA. In both, a major RNA can code for the early open reading frame E7 and a minor one can code for E6. The cervical cancer, but not the CaSki cell line, contains a minor RNA that can code for an intact E2 protein, and this may relate to the continued presence of plasmid DNA. The RNA mapping data suggest that the poly(A)+ RNA is transcribed from a minor fraction of the several hundred gene copies present, and in the cervical cancer these genomes appear to be integrated. The E7 protein has been identified in CaSki cells and the prevalence of its mRNA suggests a possible function in progression to, or long-term maintenance of, the malignant state.

Original languageEnglish (US)
Pages (from-to)4680-4684
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number13
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein'. Together they form a unique fingerprint.

  • Cite this