Transcription of functionally related constitutive genes is not coordinated

Saumil J. Gandhi, Daniel Zenklusen, Timothée Lionnet, Robert H. Singer

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Expression of an individual gene can vary considerably among genetically identical cells because of stochastic fluctuations in transcription. However, proteins comprising essential complexes or pathways have similar abundances and lower variability. It is not known whether coordination in the expression of subunits of essential complexes occurs at the level of transcription, mRNA abundance or protein expression. To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells. Our results revealed that transcript levels of temporally induced genes are highly correlated in individual cells. In contrast, transcription of constitutive genes encoding essential subunits of complexes is not coordinated because of stochastic fluctuations. The coordination of these functional complexes therefore must occur post-transcriptionally, and likely post-translationally.

Original languageEnglish (US)
Pages (from-to)27-35
Number of pages9
JournalNature Structural and Molecular Biology
Volume18
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Genes
Gene Expression
Messenger RNA
Coordination Complexes
Essential Genes
Fluorescence In Situ Hybridization
Saccharomyces cerevisiae
Proteins

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Transcription of functionally related constitutive genes is not coordinated. / Gandhi, Saumil J.; Zenklusen, Daniel; Lionnet, Timothée; Singer, Robert H.

In: Nature Structural and Molecular Biology, Vol. 18, No. 1, 01.2011, p. 27-35.

Research output: Contribution to journalArticle

Gandhi, Saumil J. ; Zenklusen, Daniel ; Lionnet, Timothée ; Singer, Robert H. / Transcription of functionally related constitutive genes is not coordinated. In: Nature Structural and Molecular Biology. 2011 ; Vol. 18, No. 1. pp. 27-35.
@article{6b074c39ab7043118f00596b7dbbc0f8,
title = "Transcription of functionally related constitutive genes is not coordinated",
abstract = "Expression of an individual gene can vary considerably among genetically identical cells because of stochastic fluctuations in transcription. However, proteins comprising essential complexes or pathways have similar abundances and lower variability. It is not known whether coordination in the expression of subunits of essential complexes occurs at the level of transcription, mRNA abundance or protein expression. To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells. Our results revealed that transcript levels of temporally induced genes are highly correlated in individual cells. In contrast, transcription of constitutive genes encoding essential subunits of complexes is not coordinated because of stochastic fluctuations. The coordination of these functional complexes therefore must occur post-transcriptionally, and likely post-translationally.",
author = "Gandhi, {Saumil J.} and Daniel Zenklusen and Timoth{\'e}e Lionnet and Singer, {Robert H.}",
year = "2011",
month = "1",
doi = "10.1038/nsmb.1934",
language = "English (US)",
volume = "18",
pages = "27--35",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Transcription of functionally related constitutive genes is not coordinated

AU - Gandhi, Saumil J.

AU - Zenklusen, Daniel

AU - Lionnet, Timothée

AU - Singer, Robert H.

PY - 2011/1

Y1 - 2011/1

N2 - Expression of an individual gene can vary considerably among genetically identical cells because of stochastic fluctuations in transcription. However, proteins comprising essential complexes or pathways have similar abundances and lower variability. It is not known whether coordination in the expression of subunits of essential complexes occurs at the level of transcription, mRNA abundance or protein expression. To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells. Our results revealed that transcript levels of temporally induced genes are highly correlated in individual cells. In contrast, transcription of constitutive genes encoding essential subunits of complexes is not coordinated because of stochastic fluctuations. The coordination of these functional complexes therefore must occur post-transcriptionally, and likely post-translationally.

AB - Expression of an individual gene can vary considerably among genetically identical cells because of stochastic fluctuations in transcription. However, proteins comprising essential complexes or pathways have similar abundances and lower variability. It is not known whether coordination in the expression of subunits of essential complexes occurs at the level of transcription, mRNA abundance or protein expression. To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells. Our results revealed that transcript levels of temporally induced genes are highly correlated in individual cells. In contrast, transcription of constitutive genes encoding essential subunits of complexes is not coordinated because of stochastic fluctuations. The coordination of these functional complexes therefore must occur post-transcriptionally, and likely post-translationally.

UR - http://www.scopus.com/inward/record.url?scp=78650967225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650967225&partnerID=8YFLogxK

U2 - 10.1038/nsmb.1934

DO - 10.1038/nsmb.1934

M3 - Article

C2 - 21131977

AN - SCOPUS:78650967225

VL - 18

SP - 27

EP - 35

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 1

ER -