Transcription of adenovirus RNA in permissive and nonpermissive infections

M. S. Farber, Stephen G. Baum

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The mechanism of blocked replication of human adenoviruses in monkey cells was examined. Previous experiments have placed the replicative block at the level of transcription or translation of adenovirus mRNA. Coinfection of the monkey cells with simian virus 40 enhances adenovirus replication in these cells. We compared the adenovirus mRNA transcribed during infection of permissive human cells and enhanced and unenhanced monkey cells. Adenovirus mRNA from enhanced monkey cells appeared to be identical to adenovirus mRNA from human cells. This indicated that simian virus 40 coinfection did not overcome the blocked replication by substituting for a missing adenovirus transcript. Comparison of adenovirus mRNA from enhanced and unenhanced monkey cell infection revealed two types of transcriptional discrepancies. There was a decrease in both the complexity and the relative abundance of several regions of the enhanced adenovirus mRNA. However, neither of these transcriptional defects was sufficient to totally explain the difference in yield of infectious virus and viral protein seen in these two types of infection.

Original languageEnglish (US)
Pages (from-to)136-148
Number of pages13
JournalJournal of Virology
Volume27
Issue number1
StatePublished - 1978

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Adenoviridae
transcription (genetics)
RNA
Haplorhini
Infection
monkeys
infection
Messenger RNA
Simian virus 40
Human Adenoviruses
cells
Coinfection
mixed infection
Protein Biosynthesis
Viral Proteins
viral proteins
translation (genetics)
Viruses
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Transcription of adenovirus RNA in permissive and nonpermissive infections. / Farber, M. S.; Baum, Stephen G.

In: Journal of Virology, Vol. 27, No. 1, 1978, p. 136-148.

Research output: Contribution to journalArticle

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