Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis

Pamela J. Mitchell, Paula M. Timmons, Jean M. Hebert, Peter W J Rigby, Robert Tjian

Research output: Contribution to journalArticle

469 Citations (Scopus)

Abstract

We have analyzed the expression pattern of transcription factor AP-2 in mouse embryos to evaluate the potential of AP-2 as a regulator during vertebrate development. A partial cDNA encoding AP-2 was isolated from a mouse embryo cDNA library and used to prepare probes to measure AP-2 mRNA levels by RNase protection and RNA in situ hybridization. Between 10.5 and 15.5 days of embryogenesis, the relative abundance of AP-2 mRNA is greatest at 11.5 days and declines steadily thereafter. RNA in situ hybridization analysis of embryos between 8.5 and 12.5 days of gestation identified a novel expression pattern for AP-2. The principle part of this expression occurs in neural crest cells and their major derivatives, including cranial and spinal sensory ganglia and facial mesenchyme. AP-2 is also expressed in surface ectoderm and in a longitudinal column of the spinal cord and hindbrain that is contacted by neural crest-derived sensory ganglia. Additional expression of AP-2 occurs in limb bud mesenchyme and in meso-metanephric regions. This embryonic expression pattern is spatially and temporally consistent with a role for AP-2 in regulating transcription of genes involved in the morphogenesis of the peripheral nervous system, face, limbs, skin, and nephric tissues.

Original languageEnglish (US)
Pages (from-to)105-119
Number of pages15
JournalGenes and Development
Volume5
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Transcription Factor AP-2
Neural Crest
Cell Lineage
Sensory Ganglia
Embryonic Development
Embryonic Structures
Mesoderm
In Situ Hybridization
RNA
Limb Buds
Rhombencephalon
Messenger RNA
Ectoderm
Peripheral Nervous System
Spinal Ganglia
Ribonucleases
Gene Library
Morphogenesis
Vertebrates
Spinal Cord

Keywords

  • AP-2
  • Mouse embryogenesis
  • Neural crest cells
  • Transcription factor
  • Vertebrate development

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Mitchell, P. J., Timmons, P. M., Hebert, J. M., Rigby, P. W. J., & Tjian, R. (1991). Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. Genes and Development, 5(1), 105-119.

Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. / Mitchell, Pamela J.; Timmons, Paula M.; Hebert, Jean M.; Rigby, Peter W J; Tjian, Robert.

In: Genes and Development, Vol. 5, No. 1, 1991, p. 105-119.

Research output: Contribution to journalArticle

Mitchell, PJ, Timmons, PM, Hebert, JM, Rigby, PWJ & Tjian, R 1991, 'Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis', Genes and Development, vol. 5, no. 1, pp. 105-119.
Mitchell, Pamela J. ; Timmons, Paula M. ; Hebert, Jean M. ; Rigby, Peter W J ; Tjian, Robert. / Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. In: Genes and Development. 1991 ; Vol. 5, No. 1. pp. 105-119.
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