Transcription cooperation by NFAT·C/EBP composite enhancer complex

Teddy T.C. Yang, Chi Wing Chow

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The nuclear factor of activated T cells (NFAT) group of transcription factors regulates gene expression in immune and non-immune cells. NFAT-mediated gene transcription is orchestrated, in part, by formation of a composite regulatory element. Here we demonstrate that NFAT interacts with transcription factor CCAAT/enhancer-binding protein (C/EBP) to form a composite enhancer complex, to potentiate expression of the peroxisome proliferator-activated receptor-γ2 gene. Formation of a ternary NFAT·C/EBP·DNA complex is required for the transcriptional cooperation. A similar NFAT·C/EBP composite element is found in the regulatory region of the insulin-like growth factor 2, angiotensin-converting enzyme homolog, and transcription factor POU4F3 genes. Thus, the NFAT·C/EBP composite element represents a novel regulatory enhancer to direct NFAT-mediated gene transcription.

Original languageEnglish (US)
Pages (from-to)15874-15885
Number of pages12
JournalJournal of Biological Chemistry
Volume278
Issue number18
DOIs
Publication statusPublished - May 2 2003

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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