Transcription cooperation by NFAT·C/EBP composite enhancer complex

Teddy T C Yang, Chi Wing Chow

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The nuclear factor of activated T cells (NFAT) group of transcription factors regulates gene expression in immune and non-immune cells. NFAT-mediated gene transcription is orchestrated, in part, by formation of a composite regulatory element. Here we demonstrate that NFAT interacts with transcription factor CCAAT/enhancer-binding protein (C/EBP) to form a composite enhancer complex, to potentiate expression of the peroxisome proliferator-activated receptor-γ2 gene. Formation of a ternary NFAT·C/EBP·DNA complex is required for the transcriptional cooperation. A similar NFAT·C/EBP composite element is found in the regulatory region of the insulin-like growth factor 2, angiotensin-converting enzyme homolog, and transcription factor POU4F3 genes. Thus, the NFAT·C/EBP composite element represents a novel regulatory enhancer to direct NFAT-mediated gene transcription.

Original languageEnglish (US)
Pages (from-to)15874-15885
Number of pages12
JournalJournal of Biological Chemistry
Volume278
Issue number18
DOIs
StatePublished - May 2 2003

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NFATC Transcription Factors
Transcription
Genes
Transcription Factors
Composite materials
CCAAT-Enhancer-Binding Proteins
Peroxisome Proliferator-Activated Receptors
Nucleic Acid Regulatory Sequences
Somatomedins
Gene expression
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transcription cooperation by NFAT·C/EBP composite enhancer complex. / Yang, Teddy T C; Chow, Chi Wing.

In: Journal of Biological Chemistry, Vol. 278, No. 18, 02.05.2003, p. 15874-15885.

Research output: Contribution to journalArticle

Yang, Teddy T C ; Chow, Chi Wing. / Transcription cooperation by NFAT·C/EBP composite enhancer complex. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 18. pp. 15874-15885.
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