Trans-heterozygous Pkd1 and Pkd2 mutations modify expression of polycystic kidney disease

Guanqing Wu, Xin Tian, Sayoko Nishimura, Glen S. Markowitz, Vivette D'Agati, Jong Hoon Park, Lili Yao, Li Li, Lin Geng, Hongyu Zhao, Winfried Edelmann, Stefan Somlo

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Abstract

Autosomal dominant polycystic kidney disease (ADPKD) occurs by germline mutation in PKD1 or PKD2. Evidence of homozygous inactivation of either gene in human cyst lining cells as well as in mouse knockout models strongly supports a two-hit mechanism for cyst formation. Discovery of trans-heterozygous mutations in PKD1 and PKD2 in a minority of human renal cysts has led to the proposal that such mutations also can play a role in cyst formation. In the current study, we investigated the role of trans-heterozygous mutations in mouse models of polycystic kidney disease. In Pkd1+/-, Pkd2+/-, and Pkd1+/-: Pkd2+/- mice, the renal cystic lesion was mild and variable with no adverse effect on survival at 1 year. In keeping with the two-hit mechanism of cyst formation, approximately 70% of kidney cysts in Pkd2+/- mice exhibited uniform loss of polycystin-2 expression. Cystic disease in trans-heterozygous Pkd1+/-: Pkd2+/- mice, however, was notable for severity in excess of that predicted by a simple additive effect based on cyst formation in singly heterozygous mice. The data suggest a modifier role for the 'trans' polycystin gene in cystic kidney disease, and support a contribution from threshold effects to cyst formation and growth.

Original languageEnglish (US)
Pages (from-to)1845-1854
Number of pages10
JournalHuman Molecular Genetics
Volume11
Issue number16
StatePublished - Aug 1 2002

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Polycystic Kidney Diseases
Cysts
Mutation
Kidney
TRPP Cation Channels
Cystic Kidney Diseases
Autosomal Dominant Polycystic Kidney
Germ-Line Mutation
Gene Silencing
Knockout Mice
Survival
Growth

ASJC Scopus subject areas

  • Genetics

Cite this

Wu, G., Tian, X., Nishimura, S., Markowitz, G. S., D'Agati, V., Park, J. H., ... Somlo, S. (2002). Trans-heterozygous Pkd1 and Pkd2 mutations modify expression of polycystic kidney disease. Human Molecular Genetics, 11(16), 1845-1854.

Trans-heterozygous Pkd1 and Pkd2 mutations modify expression of polycystic kidney disease. / Wu, Guanqing; Tian, Xin; Nishimura, Sayoko; Markowitz, Glen S.; D'Agati, Vivette; Park, Jong Hoon; Yao, Lili; Li, Li; Geng, Lin; Zhao, Hongyu; Edelmann, Winfried; Somlo, Stefan.

In: Human Molecular Genetics, Vol. 11, No. 16, 01.08.2002, p. 1845-1854.

Research output: Contribution to journalArticle

Wu, G, Tian, X, Nishimura, S, Markowitz, GS, D'Agati, V, Park, JH, Yao, L, Li, L, Geng, L, Zhao, H, Edelmann, W & Somlo, S 2002, 'Trans-heterozygous Pkd1 and Pkd2 mutations modify expression of polycystic kidney disease', Human Molecular Genetics, vol. 11, no. 16, pp. 1845-1854.
Wu G, Tian X, Nishimura S, Markowitz GS, D'Agati V, Park JH et al. Trans-heterozygous Pkd1 and Pkd2 mutations modify expression of polycystic kidney disease. Human Molecular Genetics. 2002 Aug 1;11(16):1845-1854.
Wu, Guanqing ; Tian, Xin ; Nishimura, Sayoko ; Markowitz, Glen S. ; D'Agati, Vivette ; Park, Jong Hoon ; Yao, Lili ; Li, Li ; Geng, Lin ; Zhao, Hongyu ; Edelmann, Winfried ; Somlo, Stefan. / Trans-heterozygous Pkd1 and Pkd2 mutations modify expression of polycystic kidney disease. In: Human Molecular Genetics. 2002 ; Vol. 11, No. 16. pp. 1845-1854.
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