Trans-10,cis-12-conjugated linoleic acid instigates inflammation in human adipocytes compared with preadipocytes

Kristina Martinez, Arion Kennedy, Tiffany West, Dejan Milatovic, Michael Aschner, Michael McIntosh

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We showed previously in cultures of primary human adipocytes and preadipocytes that lipopolysaccharide and trans-10,cis-12-conjugated linoleic acid (10,12-CLA) activate the inflammatory signaling that promotes insulin resistance. Because our published data demonstrated that preadipocytes are the primary instigators of inflammatory signaling in lipopolysaccharide-treated cultures, we hypothesized that they played the same role in 10,12-CLA-mediated inflammation. To test this hypothesis, we employed four distinct models. In model 1, a differentiation model, CLA activation of MAPK and induction of interleukin-8 (IL-8), IL-6, IL-1β, and cyclo-oxygenase-2 (COX-2) were greatest in differentiated compared with undifferentiated cultures. In model 2, a cell separation model, the mRNA levels of these inflammatory proteins were increased by 10,12-CLA compared with bovine serum albumin vehicle in the adipocyte fraction and the preadipocyte fraction. In model 3, a co-culture insert model, inserts containing ∼50% adipocytes (AD50) or ∼100% preadipocytes (AD0) were suspended over wells containing AD50 or AD0 cultures. 10,12-CLA-induced IL-8, IL-6, IL-1β, and COX-2 mRNA levels were highest in AD50 cultures when co-cultured with AD0 inserts. In model 4, a conditioned medium (CM) model, CM collected from CLA-treated AD50 but not AD0 cultures induced IL-8 and IL-6 mRNA levels and activated phosphorylation of MAPK in naive AD0 and AD50 cultures. Consistent with these data, 10,12-CLA-mediated secretions of IL-8 and IL-6 from AD50 cultures were higher than from AD0 cultures. Notably, blocking adipocytokine secretion prevented the inflammatory capacity of CM from 10,12-CLA-treated cultures. These data suggest that CLA instigates the release of inflammatory signals from adipocytes that subsequently activate adjacent preadipocytes.

Original languageEnglish (US)
Pages (from-to)17701-17712
Number of pages12
JournalJournal of Biological Chemistry
Volume285
Issue number23
DOIs
StatePublished - Jun 4 2010
Externally publishedYes

Fingerprint

Conjugated Linoleic Acids
Adipocytes
Interleukin-8
Inflammation
Interleukin-6
Conditioned Culture Medium
Prostaglandin-Endoperoxide Synthases
Interleukin-1
Messenger RNA
Lipopolysaccharides
Adipokines
Cell Separation
Bovine Serum Albumin
Coculture Techniques
Insulin Resistance
Phosphorylation
trans-10,cis-12-conjugated linoleic acid
Chemical activation
Proteins
Insulin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Trans-10,cis-12-conjugated linoleic acid instigates inflammation in human adipocytes compared with preadipocytes. / Martinez, Kristina; Kennedy, Arion; West, Tiffany; Milatovic, Dejan; Aschner, Michael; McIntosh, Michael.

In: Journal of Biological Chemistry, Vol. 285, No. 23, 04.06.2010, p. 17701-17712.

Research output: Contribution to journalArticle

Martinez, Kristina ; Kennedy, Arion ; West, Tiffany ; Milatovic, Dejan ; Aschner, Michael ; McIntosh, Michael. / Trans-10,cis-12-conjugated linoleic acid instigates inflammation in human adipocytes compared with preadipocytes. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 23. pp. 17701-17712.
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abstract = "We showed previously in cultures of primary human adipocytes and preadipocytes that lipopolysaccharide and trans-10,cis-12-conjugated linoleic acid (10,12-CLA) activate the inflammatory signaling that promotes insulin resistance. Because our published data demonstrated that preadipocytes are the primary instigators of inflammatory signaling in lipopolysaccharide-treated cultures, we hypothesized that they played the same role in 10,12-CLA-mediated inflammation. To test this hypothesis, we employed four distinct models. In model 1, a differentiation model, CLA activation of MAPK and induction of interleukin-8 (IL-8), IL-6, IL-1β, and cyclo-oxygenase-2 (COX-2) were greatest in differentiated compared with undifferentiated cultures. In model 2, a cell separation model, the mRNA levels of these inflammatory proteins were increased by 10,12-CLA compared with bovine serum albumin vehicle in the adipocyte fraction and the preadipocyte fraction. In model 3, a co-culture insert model, inserts containing ∼50{\%} adipocytes (AD50) or ∼100{\%} preadipocytes (AD0) were suspended over wells containing AD50 or AD0 cultures. 10,12-CLA-induced IL-8, IL-6, IL-1β, and COX-2 mRNA levels were highest in AD50 cultures when co-cultured with AD0 inserts. In model 4, a conditioned medium (CM) model, CM collected from CLA-treated AD50 but not AD0 cultures induced IL-8 and IL-6 mRNA levels and activated phosphorylation of MAPK in naive AD0 and AD50 cultures. Consistent with these data, 10,12-CLA-mediated secretions of IL-8 and IL-6 from AD50 cultures were higher than from AD0 cultures. Notably, blocking adipocytokine secretion prevented the inflammatory capacity of CM from 10,12-CLA-treated cultures. These data suggest that CLA instigates the release of inflammatory signals from adipocytes that subsequently activate adjacent preadipocytes.",
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