Traffic of p24 proteins and COPII coat composition mutually influence membrane scaffolding

Jennifer G. D'Arcangelo, Jonathan Crissman, Silvere Pagant, Alenka Čopič, Catherine F. Latham, Erik L. Snapp, Elizabeth A. Miller

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Eukaryotic protein secretion requires efficient and accurate delivery of diverse secretory and membrane proteins. This process initiates in the ER, where vesicles are sculpted by the essential COPII coat. The Sec13p subunit of the COPII coat contributes to membrane scaffolding, which enforces curvature on the nascent vesicle. A requirement for Sec13p can be bypassed when traffic of lumenally oriented membrane proteins is abrogated. Here we sought to further explore the impact of cargo proteins on vesicle formation. We show that efficient ER export of the p24 family of proteins is a major driver of the requirement for Sec13p. The scaffolding burden presented by the p24 complex is met in part by the cargo adaptor Lst1p, which binds to a subset of cargo, including the p24 proteins. We propose that the scaffolding function of Lst1p is required to generate vesicles that can accommodate difficult cargo proteins that include large oligomeric assemblies and asymmetrically distributed membrane proteins. Vesicles that contain such cargoes are also more dependent on scaffolding by Sec13p, and may serve as a model for large carrier formation in other systems.

Original languageEnglish (US)
Pages (from-to)1296-1305
Number of pages10
JournalCurrent Biology
Volume25
Issue number10
DOIs
StatePublished - May 18 2015

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Capsid Proteins
traffic
membrane proteins
Membranes
Chemical analysis
Membrane Proteins
Proteins
proteins
protein secretion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

D'Arcangelo, J. G., Crissman, J., Pagant, S., Čopič, A., Latham, C. F., Snapp, E. L., & Miller, E. A. (2015). Traffic of p24 proteins and COPII coat composition mutually influence membrane scaffolding. Current Biology, 25(10), 1296-1305. https://doi.org/10.1016/j.cub.2015.03.029

Traffic of p24 proteins and COPII coat composition mutually influence membrane scaffolding. / D'Arcangelo, Jennifer G.; Crissman, Jonathan; Pagant, Silvere; Čopič, Alenka; Latham, Catherine F.; Snapp, Erik L.; Miller, Elizabeth A.

In: Current Biology, Vol. 25, No. 10, 18.05.2015, p. 1296-1305.

Research output: Contribution to journalArticle

D'Arcangelo, JG, Crissman, J, Pagant, S, Čopič, A, Latham, CF, Snapp, EL & Miller, EA 2015, 'Traffic of p24 proteins and COPII coat composition mutually influence membrane scaffolding', Current Biology, vol. 25, no. 10, pp. 1296-1305. https://doi.org/10.1016/j.cub.2015.03.029
D'Arcangelo JG, Crissman J, Pagant S, Čopič A, Latham CF, Snapp EL et al. Traffic of p24 proteins and COPII coat composition mutually influence membrane scaffolding. Current Biology. 2015 May 18;25(10):1296-1305. https://doi.org/10.1016/j.cub.2015.03.029
D'Arcangelo, Jennifer G. ; Crissman, Jonathan ; Pagant, Silvere ; Čopič, Alenka ; Latham, Catherine F. ; Snapp, Erik L. ; Miller, Elizabeth A. / Traffic of p24 proteins and COPII coat composition mutually influence membrane scaffolding. In: Current Biology. 2015 ; Vol. 25, No. 10. pp. 1296-1305.
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