@article{6f337cf1549d4ce4a542e4380ba7f3c6,
title = "Tracing transmission of sin nombre virus and discovery of infection in multiple rodent species",
abstract = "Sin Nombre orthohantavirus (SNV), a negative-sense, single-stranded RNA virus that is carried and transmitted by the North American deer mouse Peromyscus maniculatus, can cause infection in humans through inhalation of aerosolized excreta from infected rodents. This infection can lead to hantavirus cardiopulmonary syndrome (HCPS), which has an ;36% case-fatality rate. We used reverse transcriptase quantitative PCR (RT-qPCR) to confirm SNV infection in a patient and identified SNV in lung tissues in wild-caught rodents from potential sites of exposure. Using viral whole-genome sequencing (WGS), we identified the likely site of transmission and discovered SNV in multiple rodent species not previously known to carry the virus. Here, we report, for the first time, the use of SNV WGS to pinpoint a likely site of human infection and identify SNV simultaneously in multiple rodent species in an area of known host-to-human transmission. These results will impact epidemiology and infection control for hantaviruses by tracing zoonotic transmission and investigating possible novel host reservoirs.",
keywords = "Hantavirus, Reservoir, Sequencing, Sin Nombre, Transmission",
author = "Goodfellow, {Samuel M.} and Nofchissey, {Robert A.} and Schwalm, {Kurt C.} and Cook, {Joseph A.} and Dunnum, {Jonathan L.} and Yan Guo and Chunyan Ye and Mertz, {Gregory J.} and Kartik Chandran and Michelle Harkins and Domman, {Daryl B.} and Dinwiddie, {Darrell L.} and Bradfute, {Steven B.}",
note = "Funding Information: This work was supported by a University of New Mexico School of Medicine Research Allocation Committee (UNM SOM RAC) grant (S.B.B.). S.M.G. is supported by UNM HSC Infectious Disease and Inflammation Program National Institutes of Health (NIH) grant T32AI007. Research reported in this publication was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103451 (S.M.G.). The sequencing of the patient isolates was supported by an NIH/National Institute of Allergy and Infectious Diseases (NIAID) Prometheus grant (AI-I7-042 U19). We thank the UNM Center for Advanced Research Computing, supported in part by the National Science Foundation, for providing the research computing resources used in this work. Publisher Copyright: {\textcopyright} 2021 American Society for Microbiology.",
year = "2021",
month = dec,
doi = "10.1128/JVI.01534-21",
language = "English (US)",
volume = "95",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "23",
}