Toxoplasma transcription factor TgAP2XI-5 regulates the expression of genes involved in parasite virulence and host invasion

Robert Walker, Mathieu Gissot, Ludovic Huot, Tchilabalo Dilezitoko Alayi, David Hot, Guillemette Marot, Christine Schaeffer-Reiss, Alain Van Dorsselaer, Kami Kim, Stanislas Tomavo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Gene regulation in apicomplexan parasites, a phylum containing important protozoan parasites such as Plasmodium and Toxoplasma, is poorly understood. The life cycle of Toxoplasma gondii is complex, with multiple proliferation and differentiation steps, of which tachyzoite proliferation is the most relevant to pathogenesis in humans and animals. Tachyzoites express invasion and virulence factors that are crucial for their survival and manipulation of host cell functions. The expressionofthose factors is tightly controlled during the tachyzoite cell cycle to permit their correct packaging in newly formed apical secretory organelles named micronemes and rhoptries in the daughter cells. However, little is known about the factors that control the expression of genes encoding the virulence factors present in these parasite-specific secretory organelles. We report that the plant-like nuclear factor TgAP2XI-5 targets more than 300 gene promoters and actively controls the transcriptionof these genes. Most of these target genes, including those that are essential for parasite virulence, showed a peak of expression in the S and M phases of the cell cycle. Furthermore, we identified the cisregulatory element recognized by TgAP2XI-5 and demonstrated its ability to actively drive gene transcription. Our results demonstrated that TgAP2XI-5 is a novel DNA sequence-specific transcription factor associated with promoter activation. TgAP2XI-5 may regulate gene transcription of crucial virulence factors in T. gondii.

Original languageEnglish (US)
Pages (from-to)31127-31138
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number43
DOIs
StatePublished - Oct 25 2013

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Toxoplasma
Virulence
Parasites
Transcription Factors
Genes
Gene Expression
Virulence Factors
Transcription
Organelles
Cells
Cell Cycle
Gene encoding
DNA sequences
Plasmodium
Gene expression
Product Packaging
Life cycle
Life Cycle Stages
Packaging
S Phase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Toxoplasma transcription factor TgAP2XI-5 regulates the expression of genes involved in parasite virulence and host invasion. / Walker, Robert; Gissot, Mathieu; Huot, Ludovic; Alayi, Tchilabalo Dilezitoko; Hot, David; Marot, Guillemette; Schaeffer-Reiss, Christine; Van Dorsselaer, Alain; Kim, Kami; Tomavo, Stanislas.

In: Journal of Biological Chemistry, Vol. 288, No. 43, 25.10.2013, p. 31127-31138.

Research output: Contribution to journalArticle

Walker, R, Gissot, M, Huot, L, Alayi, TD, Hot, D, Marot, G, Schaeffer-Reiss, C, Van Dorsselaer, A, Kim, K & Tomavo, S 2013, 'Toxoplasma transcription factor TgAP2XI-5 regulates the expression of genes involved in parasite virulence and host invasion', Journal of Biological Chemistry, vol. 288, no. 43, pp. 31127-31138. https://doi.org/10.1074/jbc.M113.486589
Walker, Robert ; Gissot, Mathieu ; Huot, Ludovic ; Alayi, Tchilabalo Dilezitoko ; Hot, David ; Marot, Guillemette ; Schaeffer-Reiss, Christine ; Van Dorsselaer, Alain ; Kim, Kami ; Tomavo, Stanislas. / Toxoplasma transcription factor TgAP2XI-5 regulates the expression of genes involved in parasite virulence and host invasion. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 43. pp. 31127-31138.
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