Topical application of integrin antagonists inhibits proliferative retinopathy

B. Riecke, E. Chavakis, R. G. Bretzel, T. Linn, K. T. Preissner, M. Brownlee, H. P. Hammes

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The expression of αv-integrins is highly selective for anglogenic endothelial cells; ligation inhibition by cyclic RGD peptides prevents pathological neovascularization in tumor or retinopathy models to a large extent. We have previously demonstrated that proliferative retinopathy in a mouse model of retinopathy of prematurity (ROP model) can be reduced by more than 70%. To minimize systemic side effects and unwanted interference with responsive angiogenesis, we investigated topical application of cyclic RGD-peptides. In preliminary experiments, we could exclude any inhibiting effects of the carrier solution containing EDTA, Na2S, mannitol, hydroxyethyl starch, and benzalconium chloride on the inhibitory effect of cyclic RGD peptides. Retinal presence of small molecular-mass integrin antagonists after topical application was confirmed using fluorescein-labeled cyclic RGD peptide. Topical application of the peptide to the eye inhibited proliferative retinopathy in a dose-dependent fashion with a maximum of almost 50%. These results suggest that small molecular-mass peptide antagonists of αv-type integrins are efficient in inhibiting proliferative retinopathy by topical application.

Original languageEnglish (US)
Pages (from-to)307-311
Number of pages5
JournalHormone and Metabolic Research
Volume33
Issue number5
DOIs
StatePublished - Jul 7 2001

Keywords

  • Angiogenesis
  • Diabetic retinopathy
  • RGD peptides
  • α-type integrins

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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