TonB-dependent transporter fhua in planar lipid bilayers: Partial exit of its plug from the barrel

Eshwar Udho, Karen S. Jakes, Alan Finkelstein

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

TonB-dependent transporters (TBDTs), which transport iron-chelating siderophores and vitamin B12 across the outer membrane of Gram-negative bacteria, share a conserved architecture of a 22-stranded β-barrel with an amino-terminal plug domain occluding the barrel. We previously reported that we could induce TBDTs to reversibly open in planar lipid bilayers via the use of urea and that these channels were responsive to physiological concentrations of ligands. Here we report that in the presence of urea, trypsin can cleave the amino-terminal 67 residues of the plug of the TonB-dependent transporter FhuA, as assessed by gel shift and mass spectrometry assays. On the bilayer, trypsin treatment in the presence of urea resulted in the induced conductance no longer being reversed upon removal of urea, suggesting that urea opens intact FhuA channels by pulling the plug at least partly out of the barrel and that removal of the urea then allows reinsertion of the plug into the barrel. When expressed separately, the FhuA plug domain was found to be a mostly unfolded structure that was able to occlude isolated FhuA β-barrels inserted into the membrane. Thus, although folded in the barrel, the plug need not be folded upon exiting the barrel. The rate of insertion of the β-barrels into the membrane was tremendously increased in the presence of an osmotic gradient provided by either urea or glycerol. Negative staining electron microscopy showed that FhuA in a detergent solution formed vesicles, thus explaining why an osmotic gradient promoted the insertion of FhuA into membranes.

Original languageEnglish (US)
Pages (from-to)6753-6759
Number of pages7
JournalBiochemistry
Volume51
Issue number34
DOIs
StatePublished - Aug 28 2012

Fingerprint

Lipid bilayers
Lipid Bilayers
Urea
Membranes
Trypsin
Siderophores
Negative Staining
Vitamin B 12
Chelation
Gram-Negative Bacteria
Detergents
Glycerol
Electron microscopy
Mass spectrometry
Assays
Mass Spectrometry
Electron Microscopy
Bacteria
Iron
Gels

ASJC Scopus subject areas

  • Biochemistry

Cite this

TonB-dependent transporter fhua in planar lipid bilayers : Partial exit of its plug from the barrel. / Udho, Eshwar; Jakes, Karen S.; Finkelstein, Alan.

In: Biochemistry, Vol. 51, No. 34, 28.08.2012, p. 6753-6759.

Research output: Contribution to journalArticle

Udho, Eshwar ; Jakes, Karen S. ; Finkelstein, Alan. / TonB-dependent transporter fhua in planar lipid bilayers : Partial exit of its plug from the barrel. In: Biochemistry. 2012 ; Vol. 51, No. 34. pp. 6753-6759.
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