In this chapter we review proteomic studies performed on purified postsynaptic density (PSD) fractions and analyze what their results reveal about nuclear signaling. Overall, these studies show that PSDs are similar to the cytoplasmic protein complexes found at other intercellular junctions. We find that PSDs contain numerous calmodulin (CAM)-associated transcriptional coactivators, nucleocytoplasmic shuttling proteins that bind RNA and potential splicing factors. Moreover, in conjunction with bioinformatics, we identify novel nucleocytoplasmic shuttling proteins at synapses. These results strongly suggest that control of the spatial distribution of synapse-associated nuclear factors regulates nuclear function.
|Original language||English (US)|
|Title of host publication||Transcriptional Regulation by Neuronal Activity|
|Subtitle of host publication||To the Nucleus and Back|
|Number of pages||24|
|Publication status||Published - Jan 1 2008|
ASJC Scopus subject areas