Tissue soldering by use of indocyanine green dye-enhanced fibrinogen with the near infrared diode laser

Mehmet C. Oz, Jeffrey P. Johnson, Sareh Parangi, Roy S. Chuck, Charles C. Marboe, Lawrence S. Bass, Roman Nowygrod, Michael R. Treat

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Anastomoses welded by laser have been strengthened by applying a solder of fibrinogen combined with a laser energy absorbing dye (indocyanine green, maximum absorbance 805 nm) to the anastomotic site before continuous-wave diode laser exposure (808 ± 1 nm, 4.8 W/cm2). Immediately after creation, the bursting pressures of welds created without fibrinogen (262 ± 29 mm Hg, n = 11) were significantly less than repairs with fibrinogen (330 ± 75 mm Hg, n = 11) (p < 0.05). When repairs performed with fibrinogen were exposed to urokinase (25,000 IU) the bursting pressures were not significantly different from baseline (290 ± 74 mm Hg, n = 5). Aortotomies closed by suture did not burst but leaked at pressures significantly below those of vessels closed by laser (165 ± 9 mm Hg, n = 11) (p < 0.01). Twenty-two repairs soldered with fibrinogen were incorporated into survival studies in rabbits and examined from 1 to 90 days after operation. No anastomotic ruptures, thromboses, or aneurysms were identified. Soldered sites rapidly regenerated a new intimal surface and healed by myofibroblast proliferation. No significant foreign body response was identified; the fibrinogen was resorbed. Laser soldering with exogenous fibrinogen is feasible without topical administration of additional clotting agents, significantly improves the bursting strength of primary laser welded anastomoses, and appears to result from urokinase-resistant fibrinogen cross-linking.

Original languageEnglish (US)
Pages (from-to)718-725
Number of pages8
JournalJournal of Vascular Surgery
Volume11
Issue number5
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Semiconductor Lasers
Indocyanine Green
Fibrinogen
Coloring Agents
Lasers
Urokinase-Type Plasminogen Activator
Pressure
Tunica Intima
Topical Administration
Myofibroblasts
Foreign Bodies
Sutures
Aneurysm
Rupture
Thrombosis
Rabbits

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Tissue soldering by use of indocyanine green dye-enhanced fibrinogen with the near infrared diode laser. / Oz, Mehmet C.; Johnson, Jeffrey P.; Parangi, Sareh; Chuck, Roy S.; Marboe, Charles C.; Bass, Lawrence S.; Nowygrod, Roman; Treat, Michael R.

In: Journal of Vascular Surgery, Vol. 11, No. 5, 1990, p. 718-725.

Research output: Contribution to journalArticle

Oz, Mehmet C. ; Johnson, Jeffrey P. ; Parangi, Sareh ; Chuck, Roy S. ; Marboe, Charles C. ; Bass, Lawrence S. ; Nowygrod, Roman ; Treat, Michael R. / Tissue soldering by use of indocyanine green dye-enhanced fibrinogen with the near infrared diode laser. In: Journal of Vascular Surgery. 1990 ; Vol. 11, No. 5. pp. 718-725.
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