Tissue differences in the concentration of triiodothyronine nuclear binding sites in the rat

liver, kidney, pituitary, heart, brain, spleen and testis

J. H. Oppenheimer, H. L. Schwartz, Martin I. Surks

Research output: Contribution to journalArticle

329 Citations (Scopus)

Abstract

Using a previously reported in vivo isotopic displacement technique, limited nuclear binding sites for L triiodothyronine (T3) was demonstrated in the following rat tissues: liver, kidney, heart, anterior pituitary, brain, spleen and testis. The concentration of these sites expressed both per mg DNA and per tissue (wet wt) varied widely. If liver is normalized to 1, the relative binding capacity per mg DNA is: pituitary, 1.3; liver 1.0; kidney, 0.87; heart, 0.65; brain, 0.44; spleen, 0.03; testis, 0.004. The relative binding capacity per g tissue is: pituitary, 3.7; kidney, 1.5; liver, 1.0; heart, 0.45; brain, 0.24; spleen, 0.18; and testis, 0.01. Approximately 40-50% of available sites are saturated at endogenous levels of circulating T3 and the calculated association constants of nuclear binding appear similar in the various tissues studied. The relatively low concentration of binding sites in brain, spleen, and testis is of interest since these tissues do not respond to thyroid hormone with the expected increase in oxygen consumption and in the level of mitochondrial alphaglycerophosphate dehydrogenase. A high proportion (53%) of cellular T3 in the anterior pituitary is associated with specific nuclear sites and accounts for a demonstration of limited capacity sites in unfractionated pituitary.

Original languageEnglish (US)
Pages (from-to)897-903
Number of pages7
JournalEndocrinology
Volume95
Issue number3
StatePublished - 1974

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Triiodothyronine
Testis
Spleen
Binding Sites
Kidney
Liver
Brain
Head Kidney
DNA
Thyroid Hormones
Oxygen Consumption
Oxidoreductases

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Tissue differences in the concentration of triiodothyronine nuclear binding sites in the rat : liver, kidney, pituitary, heart, brain, spleen and testis. / Oppenheimer, J. H.; Schwartz, H. L.; Surks, Martin I.

In: Endocrinology, Vol. 95, No. 3, 1974, p. 897-903.

Research output: Contribution to journalArticle

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