Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array

Edward J. Nejat, Maria Ruiz-Alonso, Carlos Simón, U. Thomas Meier

Research output: Contribution to journalArticle

6 Scopus citations


Objective: To test if nucleolar channel system (NCS) prevalence matches the accuracy of the endometrial receptivity array (ERA) for identification of the window of endometrial receptivity. Design: Comparative retrospective study, May 2008-May 2012. Setting: University-affiliated infertility clinic. Patient(s): Forty-nine healthy oocyte donors, regularly cycling, aged 20-34 years with a body mass index of 19-25 kg/m2. Intervention(s): Endometrial biopsies were collected throughout the menstrual cycle. All samples underwent transcriptomic signature identification by ERA testing (performed in a prior study) and quantification of NCS prevalence by using indirect immunofluorescence (performed in the present study). Main Outcome Measure(s): Concordance of ERA results determining the window of implantation with NCS prevalence was statistically analyzed using the kappa index. Based on dating according to the luteinizing hormone surge, specimens were dichotomized into receptive (n = 24) and nonreceptive (n = 25). The NCS prevalence was expressed as percentage of NCSs per endometrial epithelial cells in each endometrial biopsy. Result(s): Concordance of ERA and NCS dating vs. luteinizing hormone yielded comparable kappa indices of 0.878 and 0.836, respectively. Direct comparison of ERA and NCS dating resulted in a kappa index of 0.796. Conclusion(s): Prevalence of NCS identifies the window of endometrial receptivity previously identified by their transcriptomic signature using the ERA.

Original languageEnglish (US)
Pages (from-to)1477-1481
Number of pages5
JournalFertility and Sterility
Issue number5
Publication statusPublished - Jan 1 2014



  • Endometrial receptivity array (ERA)
  • Endometrium
  • Nucleolar channel system (NCS)
  • Receptivity
  • Window of implantation (WOI)

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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