Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array

Edward J. Nejat, Maria Ruiz-Alonso, Carlos Simón, U. Thomas Meier

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To test if nucleolar channel system (NCS) prevalence matches the accuracy of the endometrial receptivity array (ERA) for identification of the window of endometrial receptivity. Design: Comparative retrospective study, May 2008-May 2012. Setting: University-affiliated infertility clinic. Patient(s): Forty-nine healthy oocyte donors, regularly cycling, aged 20-34 years with a body mass index of 19-25 kg/m2. Intervention(s): Endometrial biopsies were collected throughout the menstrual cycle. All samples underwent transcriptomic signature identification by ERA testing (performed in a prior study) and quantification of NCS prevalence by using indirect immunofluorescence (performed in the present study). Main Outcome Measure(s): Concordance of ERA results determining the window of implantation with NCS prevalence was statistically analyzed using the kappa index. Based on dating according to the luteinizing hormone surge, specimens were dichotomized into receptive (n = 24) and nonreceptive (n = 25). The NCS prevalence was expressed as percentage of NCSs per endometrial epithelial cells in each endometrial biopsy. Result(s): Concordance of ERA and NCS dating vs. luteinizing hormone yielded comparable kappa indices of 0.878 and 0.836, respectively. Direct comparison of ERA and NCS dating resulted in a kappa index of 0.796. Conclusion(s): Prevalence of NCS identifies the window of endometrial receptivity previously identified by their transcriptomic signature using the ERA.

Original languageEnglish (US)
Pages (from-to)1477-1481
Number of pages5
JournalFertility and Sterility
Volume102
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Luteinizing Hormone
Biopsy
Indirect Fluorescent Antibody Technique
Menstrual Cycle
Infertility
Oocytes
Body Mass Index
Retrospective Studies
Epithelial Cells
Outcome Assessment (Health Care)
Tissue Donors

Keywords

  • Endometrial receptivity array (ERA)
  • Endometrium
  • Nucleolar channel system (NCS)
  • Receptivity
  • Window of implantation (WOI)

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array. / Nejat, Edward J.; Ruiz-Alonso, Maria; Simón, Carlos; Meier, U. Thomas.

In: Fertility and Sterility, Vol. 102, No. 5, 2014, p. 1477-1481.

Research output: Contribution to journalArticle

@article{cbe9c704dff14cc2b25b3138a14189a8,
title = "Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array",
abstract = "Objective: To test if nucleolar channel system (NCS) prevalence matches the accuracy of the endometrial receptivity array (ERA) for identification of the window of endometrial receptivity. Design: Comparative retrospective study, May 2008-May 2012. Setting: University-affiliated infertility clinic. Patient(s): Forty-nine healthy oocyte donors, regularly cycling, aged 20-34 years with a body mass index of 19-25 kg/m2. Intervention(s): Endometrial biopsies were collected throughout the menstrual cycle. All samples underwent transcriptomic signature identification by ERA testing (performed in a prior study) and quantification of NCS prevalence by using indirect immunofluorescence (performed in the present study). Main Outcome Measure(s): Concordance of ERA results determining the window of implantation with NCS prevalence was statistically analyzed using the kappa index. Based on dating according to the luteinizing hormone surge, specimens were dichotomized into receptive (n = 24) and nonreceptive (n = 25). The NCS prevalence was expressed as percentage of NCSs per endometrial epithelial cells in each endometrial biopsy. Result(s): Concordance of ERA and NCS dating vs. luteinizing hormone yielded comparable kappa indices of 0.878 and 0.836, respectively. Direct comparison of ERA and NCS dating resulted in a kappa index of 0.796. Conclusion(s): Prevalence of NCS identifies the window of endometrial receptivity previously identified by their transcriptomic signature using the ERA.",
keywords = "Endometrial receptivity array (ERA), Endometrium, Nucleolar channel system (NCS), Receptivity, Window of implantation (WOI)",
author = "Nejat, {Edward J.} and Maria Ruiz-Alonso and Carlos Sim{\'o}n and Meier, {U. Thomas}",
year = "2014",
doi = "10.1016/j.fertnstert.2014.07.1254",
language = "English (US)",
volume = "102",
pages = "1477--1481",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array

AU - Nejat, Edward J.

AU - Ruiz-Alonso, Maria

AU - Simón, Carlos

AU - Meier, U. Thomas

PY - 2014

Y1 - 2014

N2 - Objective: To test if nucleolar channel system (NCS) prevalence matches the accuracy of the endometrial receptivity array (ERA) for identification of the window of endometrial receptivity. Design: Comparative retrospective study, May 2008-May 2012. Setting: University-affiliated infertility clinic. Patient(s): Forty-nine healthy oocyte donors, regularly cycling, aged 20-34 years with a body mass index of 19-25 kg/m2. Intervention(s): Endometrial biopsies were collected throughout the menstrual cycle. All samples underwent transcriptomic signature identification by ERA testing (performed in a prior study) and quantification of NCS prevalence by using indirect immunofluorescence (performed in the present study). Main Outcome Measure(s): Concordance of ERA results determining the window of implantation with NCS prevalence was statistically analyzed using the kappa index. Based on dating according to the luteinizing hormone surge, specimens were dichotomized into receptive (n = 24) and nonreceptive (n = 25). The NCS prevalence was expressed as percentage of NCSs per endometrial epithelial cells in each endometrial biopsy. Result(s): Concordance of ERA and NCS dating vs. luteinizing hormone yielded comparable kappa indices of 0.878 and 0.836, respectively. Direct comparison of ERA and NCS dating resulted in a kappa index of 0.796. Conclusion(s): Prevalence of NCS identifies the window of endometrial receptivity previously identified by their transcriptomic signature using the ERA.

AB - Objective: To test if nucleolar channel system (NCS) prevalence matches the accuracy of the endometrial receptivity array (ERA) for identification of the window of endometrial receptivity. Design: Comparative retrospective study, May 2008-May 2012. Setting: University-affiliated infertility clinic. Patient(s): Forty-nine healthy oocyte donors, regularly cycling, aged 20-34 years with a body mass index of 19-25 kg/m2. Intervention(s): Endometrial biopsies were collected throughout the menstrual cycle. All samples underwent transcriptomic signature identification by ERA testing (performed in a prior study) and quantification of NCS prevalence by using indirect immunofluorescence (performed in the present study). Main Outcome Measure(s): Concordance of ERA results determining the window of implantation with NCS prevalence was statistically analyzed using the kappa index. Based on dating according to the luteinizing hormone surge, specimens were dichotomized into receptive (n = 24) and nonreceptive (n = 25). The NCS prevalence was expressed as percentage of NCSs per endometrial epithelial cells in each endometrial biopsy. Result(s): Concordance of ERA and NCS dating vs. luteinizing hormone yielded comparable kappa indices of 0.878 and 0.836, respectively. Direct comparison of ERA and NCS dating resulted in a kappa index of 0.796. Conclusion(s): Prevalence of NCS identifies the window of endometrial receptivity previously identified by their transcriptomic signature using the ERA.

KW - Endometrial receptivity array (ERA)

KW - Endometrium

KW - Nucleolar channel system (NCS)

KW - Receptivity

KW - Window of implantation (WOI)

UR - http://www.scopus.com/inward/record.url?scp=84922032909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922032909&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2014.07.1254

DO - 10.1016/j.fertnstert.2014.07.1254

M3 - Article

VL - 102

SP - 1477

EP - 1481

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 5

ER -