Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis

Ilme Schlichting, Steven C. Almo, Gert Rapp, Keith Wilson, Kyriakos Petratos, Arno Lentfer, Alfred Wittinghofer, Wolfgang Kabsch, Emil F. Pai, Gregory A. Petsko, Roger S. Goody

Research output: Contribution to journalArticle

393 Citations (Scopus)

Abstract

Crystals of Ha-Ras p21 with caged GTP at the active site have been used to investigate the conformational changes of p21 on GTP hydrolysis. The structure of the short-lived p21 · GTP complex was determined by Laue diffraction methods. After GTP hydrolysis, substantial structural changes occur in the parts of the molecule implicated in the interaction with GTPase-activating protein. The trigger for this process seems to be a change in coordination of the active-site Mg2+ ion as a result of loss of the γ-phosphate of GTP.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalNature
Volume345
Issue number6273
StatePublished - May 24 1990
Externally publishedYes

Fingerprint

ras Proteins
Guanosine Triphosphate
Hydrolysis
X-Rays
Catalytic Domain
Proto-Oncogene Proteins p21(ras)
GTPase-Activating Proteins
Phosphates
Ions

ASJC Scopus subject areas

  • General

Cite this

Schlichting, I., Almo, S. C., Rapp, G., Wilson, K., Petratos, K., Lentfer, A., ... Goody, R. S. (1990). Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis. Nature, 345(6273), 309-315.

Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis. / Schlichting, Ilme; Almo, Steven C.; Rapp, Gert; Wilson, Keith; Petratos, Kyriakos; Lentfer, Arno; Wittinghofer, Alfred; Kabsch, Wolfgang; Pai, Emil F.; Petsko, Gregory A.; Goody, Roger S.

In: Nature, Vol. 345, No. 6273, 24.05.1990, p. 309-315.

Research output: Contribution to journalArticle

Schlichting, I, Almo, SC, Rapp, G, Wilson, K, Petratos, K, Lentfer, A, Wittinghofer, A, Kabsch, W, Pai, EF, Petsko, GA & Goody, RS 1990, 'Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis', Nature, vol. 345, no. 6273, pp. 309-315.
Schlichting I, Almo SC, Rapp G, Wilson K, Petratos K, Lentfer A et al. Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis. Nature. 1990 May 24;345(6273):309-315.
Schlichting, Ilme ; Almo, Steven C. ; Rapp, Gert ; Wilson, Keith ; Petratos, Kyriakos ; Lentfer, Arno ; Wittinghofer, Alfred ; Kabsch, Wolfgang ; Pai, Emil F. ; Petsko, Gregory A. ; Goody, Roger S. / Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis. In: Nature. 1990 ; Vol. 345, No. 6273. pp. 309-315.
@article{90ad4c9e16fe4ccabdbf555107e7d29f,
title = "Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis",
abstract = "Crystals of Ha-Ras p21 with caged GTP at the active site have been used to investigate the conformational changes of p21 on GTP hydrolysis. The structure of the short-lived p21 · GTP complex was determined by Laue diffraction methods. After GTP hydrolysis, substantial structural changes occur in the parts of the molecule implicated in the interaction with GTPase-activating protein. The trigger for this process seems to be a change in coordination of the active-site Mg2+ ion as a result of loss of the γ-phosphate of GTP.",
author = "Ilme Schlichting and Almo, {Steven C.} and Gert Rapp and Keith Wilson and Kyriakos Petratos and Arno Lentfer and Alfred Wittinghofer and Wolfgang Kabsch and Pai, {Emil F.} and Petsko, {Gregory A.} and Goody, {Roger S.}",
year = "1990",
month = "5",
day = "24",
language = "English (US)",
volume = "345",
pages = "309--315",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6273",

}

TY - JOUR

T1 - Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis

AU - Schlichting, Ilme

AU - Almo, Steven C.

AU - Rapp, Gert

AU - Wilson, Keith

AU - Petratos, Kyriakos

AU - Lentfer, Arno

AU - Wittinghofer, Alfred

AU - Kabsch, Wolfgang

AU - Pai, Emil F.

AU - Petsko, Gregory A.

AU - Goody, Roger S.

PY - 1990/5/24

Y1 - 1990/5/24

N2 - Crystals of Ha-Ras p21 with caged GTP at the active site have been used to investigate the conformational changes of p21 on GTP hydrolysis. The structure of the short-lived p21 · GTP complex was determined by Laue diffraction methods. After GTP hydrolysis, substantial structural changes occur in the parts of the molecule implicated in the interaction with GTPase-activating protein. The trigger for this process seems to be a change in coordination of the active-site Mg2+ ion as a result of loss of the γ-phosphate of GTP.

AB - Crystals of Ha-Ras p21 with caged GTP at the active site have been used to investigate the conformational changes of p21 on GTP hydrolysis. The structure of the short-lived p21 · GTP complex was determined by Laue diffraction methods. After GTP hydrolysis, substantial structural changes occur in the parts of the molecule implicated in the interaction with GTPase-activating protein. The trigger for this process seems to be a change in coordination of the active-site Mg2+ ion as a result of loss of the γ-phosphate of GTP.

UR - http://www.scopus.com/inward/record.url?scp=0025275582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025275582&partnerID=8YFLogxK

M3 - Article

VL - 345

SP - 309

EP - 315

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6273

ER -