This chapter focuses on the use of time-resolved resonance Raman scattering as a tool to address specific questions pertaining to how hemoglobin (Hb) functions. Multichannel detectors have replaced photomultiplier tubes as the detectors of choice for most time-resolved Raman studies. These detectors allow for monitoring an entire segment of the Raman spectrum at a single setting of a spectrograph. Intensified diode array detectors can be gated on the 5-nsec and slower time scale. The gating capability allows for discrimination between two temporally distinct signals, including Raman or luminescence generated from the excitation pulse and the probe pulse. Geminate recombination plays a pivotal role in understanding how structure controls ligand binding in Hb. This significance stems both from the relative simplicity of the process and from the indications that protein control of ligand binding occurs primarily at the level of the bond-forming. The chapter discusses the technology connected with this form of spectroscopy and how this technology is being applied to specific Hb-related problems.
ASJC Scopus subject areas
- Molecular Biology