Time-resolved hydroxyl radical footprinting of RNA with X-rays.

S. A. Woodson; M. L. Deras; M. Brenowitz

doi:10.1002/0471142700.nc1106s06

Time-resolved hydroxyl radical footprinting of RNA with X-rays.

S. A. Woodson, M. L. Deras, M. Brenowitz

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

RNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X-ray beam provides analysis on a short timescale (50 msec to 100 sec), which enables the structures of folding intermediates or other transient conformational states to be determined. This unit provides detailed instructions on the use of the synchrotron beamline for hydroxyl radical footprinting.

Original language	English (US)
Pages (from-to)	Unit 11.6
Journal	Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
Volume	Chapter 11
DOIs	https://doi.org/10.1002/0471142700.nc1106s06
State	Published - Nov 2001
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

Access to Document

10.1002/0471142700.nc1106s06

Cite this

@article{ce062942a7384c1aab9eff2869f205ea,

title = "Time-resolved hydroxyl radical footprinting of RNA with X-rays.",

abstract = "RNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X-ray beam provides analysis on a short timescale (50 msec to 100 sec), which enables the structures of folding intermediates or other transient conformational states to be determined. This unit provides detailed instructions on the use of the synchrotron beamline for hydroxyl radical footprinting.",

author = "Woodson, {S. A.} and Deras, {M. L.} and M. Brenowitz",

year = "2001",

month = nov,

doi = "10.1002/0471142700.nc1106s06",

language = "English (US)",

volume = "Chapter 11",

pages = "Unit 11.6",

journal = "Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]",

issn = "1934-9270",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Time-resolved hydroxyl radical footprinting of RNA with X-rays.

AU - Woodson, S. A.

AU - Deras, M. L.

AU - Brenowitz, M.

PY - 2001/11

Y1 - 2001/11

N2 - RNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X-ray beam provides analysis on a short timescale (50 msec to 100 sec), which enables the structures of folding intermediates or other transient conformational states to be determined. This unit provides detailed instructions on the use of the synchrotron beamline for hydroxyl radical footprinting.

AB - RNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X-ray beam provides analysis on a short timescale (50 msec to 100 sec), which enables the structures of folding intermediates or other transient conformational states to be determined. This unit provides detailed instructions on the use of the synchrotron beamline for hydroxyl radical footprinting.

UR - http://www.scopus.com/inward/record.url?scp=43049170387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43049170387&partnerID=8YFLogxK

U2 - 10.1002/0471142700.nc1106s06

DO - 10.1002/0471142700.nc1106s06

M3 - Article

C2 - 18428832

AN - SCOPUS:43049170387

SN - 1934-9270

VL - Chapter 11

SP - Unit 11.6

JO - Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]

JF - Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]

ER -

Time-resolved hydroxyl radical footprinting of RNA with X-rays.

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this