Time-lapse imaging of membrane traffic in living cells

Erik Lee Snapp, Patrick Lajoie

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems. This protocol describes the use of the confocal laser-scanning microscope (CLSM) for time-lapse imaging of one or more fluorescent markers.

Original languageEnglish (US)
Pages (from-to)1362-1365
Number of pages4
JournalCold Spring Harbor Protocols
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 1 2011

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this