Time-lapse imaging of membrane traffic in living cells

Erik Lee Snapp; Patrick Lajoie

doi:10.1101/pdb.prot066555

Time-lapse imaging of membrane traffic in living cells

Erik Lee Snapp, Patrick Lajoie

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems. This protocol describes the use of the confocal laser-scanning microscope (CLSM) for time-lapse imaging of one or more fluorescent markers.

Original language	English (US)
Pages (from-to)	1362-1365
Number of pages	4
Journal	Cold Spring Harbor Protocols
Volume	6
Issue number	11
DOIs	https://doi.org/10.1101/pdb.prot066555
State	Published - Nov 2011
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1101/pdb.prot066555

Cite this

@article{7b9cfe4ffb404ce49866e3440d1c5540,

title = "Time-lapse imaging of membrane traffic in living cells",

abstract = "Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems. This protocol describes the use of the confocal laser-scanning microscope (CLSM) for time-lapse imaging of one or more fluorescent markers.",

author = "Snapp, {Erik Lee} and Patrick Lajoie",

year = "2011",

month = nov,

doi = "10.1101/pdb.prot066555",

language = "English (US)",

volume = "6",

pages = "1362--1365",

journal = "Cold Spring Harbor Protocols",

issn = "1559-6095",

publisher = "Cold Spring Harbor Laboratory Press",

number = "11",

}

TY - JOUR

T1 - Time-lapse imaging of membrane traffic in living cells

AU - Snapp, Erik Lee

AU - Lajoie, Patrick

PY - 2011/11

Y1 - 2011/11

N2 - Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems. This protocol describes the use of the confocal laser-scanning microscope (CLSM) for time-lapse imaging of one or more fluorescent markers.

AB - Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems. This protocol describes the use of the confocal laser-scanning microscope (CLSM) for time-lapse imaging of one or more fluorescent markers.

UR - http://www.scopus.com/inward/record.url?scp=80455174553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80455174553&partnerID=8YFLogxK

U2 - 10.1101/pdb.prot066555

DO - 10.1101/pdb.prot066555

M3 - Article

C2 - 22046037

AN - SCOPUS:80455174553

SN - 1559-6095

VL - 6

SP - 1362

EP - 1365

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 11

ER -

Time-lapse imaging of membrane traffic in living cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this