Time-lapse imaging of membrane traffic in living cells

Erik Lee Snapp, Patrick Lajoie

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments-including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes-that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being studied in living cells because of the availability of green fluorescent protein (GFP)- fusion proteins and recent advances in fluorescent microscope imaging systems. This protocol describes the use of the confocal laser-scanning microscope (CLSM) for time-lapse imaging of one or more fluorescent markers.

Original languageEnglish (US)
Pages (from-to)1362-1365
Number of pages4
JournalCold Spring Harbor Protocols
Volume6
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

Time-Lapse Imaging
Microscopes
Cells
Membranes
Imaging techniques
Caveolae
Mitochondria
Peroxisomes
Endosomes
Nuclear Envelope
Golgi Apparatus
Eukaryotic Cells
Green Fluorescent Proteins
Lysosomes
Endoplasmic Reticulum
Imaging systems
Lasers
Fusion reactions
Availability
Scanning

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Time-lapse imaging of membrane traffic in living cells. / Snapp, Erik Lee; Lajoie, Patrick.

In: Cold Spring Harbor Protocols, Vol. 6, No. 11, 11.2011, p. 1362-1365.

Research output: Contribution to journalArticle

Snapp, Erik Lee ; Lajoie, Patrick. / Time-lapse imaging of membrane traffic in living cells. In: Cold Spring Harbor Protocols. 2011 ; Vol. 6, No. 11. pp. 1362-1365.
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