Tie1 is required for lymphatic valve and collecting vessel development

Xianghu Qu, Bin Zhou, H. Scott Baldwin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tie1 is a receptor tyrosine kinase with broad expression in embryonic endothelium. Reduction of Tie1 levels in mouse embryos with a hypomorphic Tie1 allele resulted in abnormal lymphatic patterning and architecture, decreased lymphatic draining efficiency, and ultimately, embryonic demise. Here we report that Tie1 is present uniformly throughout the lymphatics and from late embryonic/early postnatal stages, becomes more restricted to lymphatic valve regions. To investigate later events of lymphatic development, we employed Cre- loxP recombination utilizing a floxed Tie1 allele and an Nfatc1Cre line, to provide loxP excision predominantly in lymphatic endothelium and developing valves. Interestingly, unlike the early prenatal defects previously described by ubiquitous endothelial deletion, excision of Tie1 with Nfatc1Cre resulted in abnormal lymphatic defects in postnatal mice and was characterized by agenesis of lymphatic valves and a deficiency of collecting lymphatic vessels. Attenuation of Tie1 signaling in lymphatic endothelium prevented initiation of lymphatic valve specification by Prox1 high expression lymphatic endothelial cells that is associated with the onset of turbulent flow in the lymphatic circulation. Our findings reveal a fundamental role for Tie1 signaling during lymphatic vessel remodeling and valve morphogenesis and implicate it as a candidate gene involved in primary lymphedema.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalDevelopmental Biology
Volume399
Issue number1
DOIs
StatePublished - Mar 1 2015

Fingerprint

Lymphatic Endothelium
Lymphatic Vessels
Alleles
Lymphedema
Receptor Protein-Tyrosine Kinases
Morphogenesis
Genetic Recombination
Endothelium
Embryonic Structures
Endothelial Cells
Genes

Keywords

  • Conditional knockout
  • Lymphatic valve
  • Lymphatic vessel remodeling
  • Lymphovenous valve
  • Prox1
  • Tie1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Tie1 is required for lymphatic valve and collecting vessel development. / Qu, Xianghu; Zhou, Bin; Scott Baldwin, H.

In: Developmental Biology, Vol. 399, No. 1, 01.03.2015, p. 117-128.

Research output: Contribution to journalArticle

Qu, Xianghu ; Zhou, Bin ; Scott Baldwin, H. / Tie1 is required for lymphatic valve and collecting vessel development. In: Developmental Biology. 2015 ; Vol. 399, No. 1. pp. 117-128.
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