Tibial fracture decreases oxygen levels at the site of injury.

Chuanyong Lu, Mark Rollins, Huagang Hou, Harold M. Swartz, Harriet Hopf, Theodore Miclau, Ralph S. Marcucio

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

OBJECTIVES: Oxygen is an essential component for many aspects of tissue repair. However, the effect of oxygen levels on differentiation of stem cells into osteoblasts and chondrocytes during fracture healing is unknown, in part because of the difficulty in measuring oxygen during fracture healing. In this study we tested the feasibility of using electron paramagnetic resonance (EPR) oximetry to assess tissue oxygen partial pressure (pO(2)) after tibial fractures in mice. METHODS: Transverse tibia fractures were created by three-point bending in adult mice. Paramagnetic material, lithium phthalocyanine (LiPc), was implanted into the fracture site or adjacent to the periosteum in the contralateral leg immediately after fracture. Tissue pO(2) was assessed by EPR 90-110 minutes after implantation of the crystals. in a second experiment, LiPc was implanted into the fracture site and fracture repair and the bio-compatibility of LiPc were assessed at 14 and 28 days after injury. RESULTS: At the very early stage after fracture, injury significantly decreased tissue oxygenation at the fracture site. When animals were breathing 21% oxygen, pO(2) at the fracture site ((30.6 +/- 12.7 mmHg, n=7) was lower than that in contralateral legs (45.5 +/- 15.3 mmHg, n=7, p<0.01). breathing 100% inspired oxygen increased the pO(2) in both the fractured (72.8 +/- 28.2 mmHg; n=7) and contralateral legs (148.4 +/- 59.2 mmHg; n=7, p<0.01). in addition, LiPc crystals implanted into fracture sites did not interfere with normal fracture healing at 10 and 28 days post-injury. CONCLUSIONS: EPR oximetry is a valuable tool for monitoring oxygen levels during fracture repair in mice.

Original languageEnglish (US)
Pages (from-to)14-21
Number of pages8
JournalThe Iowa orthopaedic journal
Volume28
StatePublished - 2008
Externally publishedYes

Fingerprint

Tibial Fractures
Oxygen
Wounds and Injuries
Fracture Healing
Electron Spin Resonance Spectroscopy
Leg
Oximetry
Respiration
Periosteum
Partial Pressure
Feasibility Studies
Chondrocytes
Osteoblasts
Tibia
Stem Cells
lithium phthalocyanine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lu, C., Rollins, M., Hou, H., Swartz, H. M., Hopf, H., Miclau, T., & Marcucio, R. S. (2008). Tibial fracture decreases oxygen levels at the site of injury. The Iowa orthopaedic journal, 28, 14-21.

Tibial fracture decreases oxygen levels at the site of injury. / Lu, Chuanyong; Rollins, Mark; Hou, Huagang; Swartz, Harold M.; Hopf, Harriet; Miclau, Theodore; Marcucio, Ralph S.

In: The Iowa orthopaedic journal, Vol. 28, 2008, p. 14-21.

Research output: Contribution to journalArticle

Lu, C, Rollins, M, Hou, H, Swartz, HM, Hopf, H, Miclau, T & Marcucio, RS 2008, 'Tibial fracture decreases oxygen levels at the site of injury.', The Iowa orthopaedic journal, vol. 28, pp. 14-21.
Lu C, Rollins M, Hou H, Swartz HM, Hopf H, Miclau T et al. Tibial fracture decreases oxygen levels at the site of injury. The Iowa orthopaedic journal. 2008;28:14-21.
Lu, Chuanyong ; Rollins, Mark ; Hou, Huagang ; Swartz, Harold M. ; Hopf, Harriet ; Miclau, Theodore ; Marcucio, Ralph S. / Tibial fracture decreases oxygen levels at the site of injury. In: The Iowa orthopaedic journal. 2008 ; Vol. 28. pp. 14-21.
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title = "Tibial fracture decreases oxygen levels at the site of injury.",
abstract = "OBJECTIVES: Oxygen is an essential component for many aspects of tissue repair. However, the effect of oxygen levels on differentiation of stem cells into osteoblasts and chondrocytes during fracture healing is unknown, in part because of the difficulty in measuring oxygen during fracture healing. In this study we tested the feasibility of using electron paramagnetic resonance (EPR) oximetry to assess tissue oxygen partial pressure (pO(2)) after tibial fractures in mice. METHODS: Transverse tibia fractures were created by three-point bending in adult mice. Paramagnetic material, lithium phthalocyanine (LiPc), was implanted into the fracture site or adjacent to the periosteum in the contralateral leg immediately after fracture. Tissue pO(2) was assessed by EPR 90-110 minutes after implantation of the crystals. in a second experiment, LiPc was implanted into the fracture site and fracture repair and the bio-compatibility of LiPc were assessed at 14 and 28 days after injury. RESULTS: At the very early stage after fracture, injury significantly decreased tissue oxygenation at the fracture site. When animals were breathing 21{\%} oxygen, pO(2) at the fracture site ((30.6 +/- 12.7 mmHg, n=7) was lower than that in contralateral legs (45.5 +/- 15.3 mmHg, n=7, p<0.01). breathing 100{\%} inspired oxygen increased the pO(2) in both the fractured (72.8 +/- 28.2 mmHg; n=7) and contralateral legs (148.4 +/- 59.2 mmHg; n=7, p<0.01). in addition, LiPc crystals implanted into fracture sites did not interfere with normal fracture healing at 10 and 28 days post-injury. CONCLUSIONS: EPR oximetry is a valuable tool for monitoring oxygen levels during fracture repair in mice.",
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T1 - Tibial fracture decreases oxygen levels at the site of injury.

AU - Lu, Chuanyong

AU - Rollins, Mark

AU - Hou, Huagang

AU - Swartz, Harold M.

AU - Hopf, Harriet

AU - Miclau, Theodore

AU - Marcucio, Ralph S.

PY - 2008

Y1 - 2008

N2 - OBJECTIVES: Oxygen is an essential component for many aspects of tissue repair. However, the effect of oxygen levels on differentiation of stem cells into osteoblasts and chondrocytes during fracture healing is unknown, in part because of the difficulty in measuring oxygen during fracture healing. In this study we tested the feasibility of using electron paramagnetic resonance (EPR) oximetry to assess tissue oxygen partial pressure (pO(2)) after tibial fractures in mice. METHODS: Transverse tibia fractures were created by three-point bending in adult mice. Paramagnetic material, lithium phthalocyanine (LiPc), was implanted into the fracture site or adjacent to the periosteum in the contralateral leg immediately after fracture. Tissue pO(2) was assessed by EPR 90-110 minutes after implantation of the crystals. in a second experiment, LiPc was implanted into the fracture site and fracture repair and the bio-compatibility of LiPc were assessed at 14 and 28 days after injury. RESULTS: At the very early stage after fracture, injury significantly decreased tissue oxygenation at the fracture site. When animals were breathing 21% oxygen, pO(2) at the fracture site ((30.6 +/- 12.7 mmHg, n=7) was lower than that in contralateral legs (45.5 +/- 15.3 mmHg, n=7, p<0.01). breathing 100% inspired oxygen increased the pO(2) in both the fractured (72.8 +/- 28.2 mmHg; n=7) and contralateral legs (148.4 +/- 59.2 mmHg; n=7, p<0.01). in addition, LiPc crystals implanted into fracture sites did not interfere with normal fracture healing at 10 and 28 days post-injury. CONCLUSIONS: EPR oximetry is a valuable tool for monitoring oxygen levels during fracture repair in mice.

AB - OBJECTIVES: Oxygen is an essential component for many aspects of tissue repair. However, the effect of oxygen levels on differentiation of stem cells into osteoblasts and chondrocytes during fracture healing is unknown, in part because of the difficulty in measuring oxygen during fracture healing. In this study we tested the feasibility of using electron paramagnetic resonance (EPR) oximetry to assess tissue oxygen partial pressure (pO(2)) after tibial fractures in mice. METHODS: Transverse tibia fractures were created by three-point bending in adult mice. Paramagnetic material, lithium phthalocyanine (LiPc), was implanted into the fracture site or adjacent to the periosteum in the contralateral leg immediately after fracture. Tissue pO(2) was assessed by EPR 90-110 minutes after implantation of the crystals. in a second experiment, LiPc was implanted into the fracture site and fracture repair and the bio-compatibility of LiPc were assessed at 14 and 28 days after injury. RESULTS: At the very early stage after fracture, injury significantly decreased tissue oxygenation at the fracture site. When animals were breathing 21% oxygen, pO(2) at the fracture site ((30.6 +/- 12.7 mmHg, n=7) was lower than that in contralateral legs (45.5 +/- 15.3 mmHg, n=7, p<0.01). breathing 100% inspired oxygen increased the pO(2) in both the fractured (72.8 +/- 28.2 mmHg; n=7) and contralateral legs (148.4 +/- 59.2 mmHg; n=7, p<0.01). in addition, LiPc crystals implanted into fracture sites did not interfere with normal fracture healing at 10 and 28 days post-injury. CONCLUSIONS: EPR oximetry is a valuable tool for monitoring oxygen levels during fracture repair in mice.

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