TIAM-1/GEF can shape somatosensory dendrites independently of its GEF activity by regulating F-actin localization

Leo Th Tang, Carlos A. Diaz-Balzac, Maisha Rahman, Nelson J. Ramirez-Suarez, Yehuda Salzberg, Maria I. Lázaro-Peña, Hannes E. Buelow

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dendritic arbors are crucial for nervous system assembly, but the intracellular mechanisms that govern their assembly remain incompletely understood. Here, we show that the dendrites of PVD neurons in Caenorhabditis elegans are patterned by distinct pathways downstream of the DMA-1 leucine-rich transmembrane (LRR-TM) receptor. DMA-1/LRR-TM interacts through a PDZ ligand motif with the guanine nucleotide exchange factor TIAM-1/GEF in a complex with act-4/Actin to pattern higher order 4° dendrite branches by localizing F-actin to the distal ends of developing dendrites. Surprisingly, TIAM-1/GEF appears to function independently of Rac1 guanine nucleotide exchange factor activity. A partially redundant pathway, dependent on HPO-30/Claudin, regulates formation of 2° and 3° branches, possibly by regulating membrane localization and trafficking of DMA-1/LRR-TM. Collectively, our experiments suggest that HPO-30/Claudin localizes the DMA-1/LRR-TM receptor on PVD dendrites, which in turn can control dendrite patterning by directly modulating F-actin dynamics through TIAM-1/GEF.

Original languageEnglish (US)
JournaleLife
Volume8
DOIs
StatePublished - Jan 29 2019

Fingerprint

Dynamic mechanical analysis
Dendrites
Actins
Guanine Nucleotide Exchange Factors
Physical vapor deposition
Neurology
Leucine
Neurons
Caenorhabditis elegans
Nervous System
Ligands
Membranes
Experiments
TM 1

Keywords

  • actin
  • C. elegans
  • claudin
  • dendrites
  • developmental biology
  • GEF independent
  • guanine nucleotide exchange factor
  • neuroscience
  • PVD

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Tang, L. T., Diaz-Balzac, C. A., Rahman, M., Ramirez-Suarez, N. J., Salzberg, Y., Lázaro-Peña, M. I., & Buelow, H. E. (2019). TIAM-1/GEF can shape somatosensory dendrites independently of its GEF activity by regulating F-actin localization. eLife, 8. https://doi.org/10.7554/eLife.38949

TIAM-1/GEF can shape somatosensory dendrites independently of its GEF activity by regulating F-actin localization. / Tang, Leo Th; Diaz-Balzac, Carlos A.; Rahman, Maisha; Ramirez-Suarez, Nelson J.; Salzberg, Yehuda; Lázaro-Peña, Maria I.; Buelow, Hannes E.

In: eLife, Vol. 8, 29.01.2019.

Research output: Contribution to journalArticle

Tang LT, Diaz-Balzac CA, Rahman M, Ramirez-Suarez NJ, Salzberg Y, Lázaro-Peña MI et al. TIAM-1/GEF can shape somatosensory dendrites independently of its GEF activity by regulating F-actin localization. eLife. 2019 Jan 29;8. https://doi.org/10.7554/eLife.38949
Tang, Leo Th ; Diaz-Balzac, Carlos A. ; Rahman, Maisha ; Ramirez-Suarez, Nelson J. ; Salzberg, Yehuda ; Lázaro-Peña, Maria I. ; Buelow, Hannes E. / TIAM-1/GEF can shape somatosensory dendrites independently of its GEF activity by regulating F-actin localization. In: eLife. 2019 ; Vol. 8.
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