Abstract
Using an immunoprecipitation-reverse transcription-PCR technique, we characterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS protein and the mRNA of the nuclear oncogene c-myc. TS protein is complexed in intact cells with the C-terminal coding region of c-myc mRNA that includes nucleotide positions 1625 to 1790. RNA electrophoretic gel mobility shift assays confirm a specific interaction between TS protein and c-myc mRNA and provide additional evidence that the C-terminal coding region represents an important cis- acting regulatory element. Further evidence demonstrates that the in vitro translational efficiency of c-myc mRNA is inhibited as a result of its direct interaction with TS protein. In addition, the presence of exogenous c-myc mRNA specifically relieves the inhibitory effects of TS protein on TS mRNA translation.
Original language | English (US) |
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Pages (from-to) | 179-185 |
Number of pages | 7 |
Journal | Molecular and cellular biology |
Volume | 15 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1995 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology