Thy/liv-SCID-hu mice implanted with human intestine: An in vivo model for investigation of mucosal transmission of HIV

Chris Gibbons, Tobias R. Kollmann, Massimo Pettoello-Mantovani, Ana Kim, Harris Goldstein

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mucosal transmission is a major route by which individuals become infected with HIV. Investigation into the mechanism by which mucosal transmission of HIV occurs would be greatly facilitated by the development of a small animal model infectible with HIV by the mucosal route. We have previously described a SCID-hu mouse model, in which human thymic and liver tissues are implanted under both kidney capsules (thy/liv-SCID-hu mice), which are populated in the periphery with high numbers of human T cells and that develop disseminated HIV-1 infection after intraperitoneal injection. To expand further the usefulness of the thy/liv-SCID-hu mouse as a model for studying mucosal transmission of HIV, thy/liv-SCID-hu mice were subcutaneously implanted with human intestinal tissue in a manner that maintained the lumen. Four months later, the histological appearance of the implanted intestine resembled that of normal human bowel tissue and the lamina propria was populated with human T cells. Six weeks after introduction of HIV into the lumen of the intestinal implant, the mice developed disseminated HIV infection. Scattered HIV-infected cells were detected in the lamina propria of the implant, indicating that HIV infection in these mice was mediated by transmission of the virus across the mucosa of the human intestinal implant. Thus, our modified thy/liv-SCID-hu mice transplanted with human bowel tissue should provide a novel model for investigating mucosal transmission of HIV.

Original languageEnglish (US)
Pages (from-to)1453-1460
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume13
Issue number17
StatePublished - Nov 20 1997

Fingerprint

SCID Mice
Intestines
HIV
HIV Infections
Mucous Membrane
T-Lymphocytes
Intestinal Mucosa
Intraperitoneal Injections
Capsules
HIV-1
Animal Models
Viruses
Kidney
Liver

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Thy/liv-SCID-hu mice implanted with human intestine : An in vivo model for investigation of mucosal transmission of HIV. / Gibbons, Chris; Kollmann, Tobias R.; Pettoello-Mantovani, Massimo; Kim, Ana; Goldstein, Harris.

In: AIDS Research and Human Retroviruses, Vol. 13, No. 17, 20.11.1997, p. 1453-1460.

Research output: Contribution to journalArticle

Gibbons, Chris ; Kollmann, Tobias R. ; Pettoello-Mantovani, Massimo ; Kim, Ana ; Goldstein, Harris. / Thy/liv-SCID-hu mice implanted with human intestine : An in vivo model for investigation of mucosal transmission of HIV. In: AIDS Research and Human Retroviruses. 1997 ; Vol. 13, No. 17. pp. 1453-1460.
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