thy/liv-SCID-hu mice: A system for investigating the in vivo effects of multidrug therapy on plasma viremia and human immunodeficiency virus replication in lymphoid tissues

Massimo Pettoello-Mantovani, Tobias R. Kollmann, Nikos F. Katopodis, Christina Raker, Ana Kim, Sergey Yurasov, Hugh Wiltshire, Harris Goldstein

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Modified, human immunodeficiency virus (HIV)-inoculated thy/liv-SCID-hu mice were used to evaluate the in vivo efficacy of antiretroviral drugs. Ritonavir treatment alone initially suppressed plasma viremia, but the viremia recurred with the appearance of ritonavir-resistant HIV isolates. Multidrug therapy suppressed plasma HIV RNA to undetectable levels; however, plasma viremia returned after therapy was stopped, showing that the therapy did not completely suppress HIV infection in the thymic implant. When thy/liv-SCID-hu mice were treated with a combination of zidovudine, lamivudine, and ritonavir immediately after inoculation with HIV, cocultures of the thymic implants remained negative for HIV even 1 month after therapy was discontinued, suggesting that acute treatment can prevent the establishment of HIV infection. Thus, these modified thy/liv-SCID-hu mice should prove to be a useful system for evaluating the effectiveness of different antiretroviral therapies on acute and chronic HIV infection.

Original languageEnglish (US)
Pages (from-to)337-346
Number of pages10
JournalJournal of Infectious Diseases
Volume177
Issue number2
StatePublished - 1998

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SCID Mice
Viremia
Lymphoid Tissue
Virus Replication
HIV
Ritonavir
Virus Diseases
Therapeutics
Lamivudine
Zidovudine
Coculture Techniques
HIV-1
RNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

thy/liv-SCID-hu mice : A system for investigating the in vivo effects of multidrug therapy on plasma viremia and human immunodeficiency virus replication in lymphoid tissues. / Pettoello-Mantovani, Massimo; Kollmann, Tobias R.; Katopodis, Nikos F.; Raker, Christina; Kim, Ana; Yurasov, Sergey; Wiltshire, Hugh; Goldstein, Harris.

In: Journal of Infectious Diseases, Vol. 177, No. 2, 1998, p. 337-346.

Research output: Contribution to journalArticle

Pettoello-Mantovani, Massimo ; Kollmann, Tobias R. ; Katopodis, Nikos F. ; Raker, Christina ; Kim, Ana ; Yurasov, Sergey ; Wiltshire, Hugh ; Goldstein, Harris. / thy/liv-SCID-hu mice : A system for investigating the in vivo effects of multidrug therapy on plasma viremia and human immunodeficiency virus replication in lymphoid tissues. In: Journal of Infectious Diseases. 1998 ; Vol. 177, No. 2. pp. 337-346.
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