Three-stage assembly of the cysteine synthase complex from Escherichia coli

Ting Wang, Thomas S. Leyh

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Control of sulfur metabolism in plants and bacteria is linked, in significant measure, to the behavior of the cysteine synthase complex (CSC). The complex is comprised of the two enzymes that catalyze the final steps in cysteine biosynthesis: serine O-acetyltransferase (SAT, EC 2.3.1.30), which produces O-acetyl-L-serine, and O-acetyl-L-serine sulfhydrylase (OASS, EC 2.5.1.47), which converts it to cysteine. SAT (a dimer of homotrimers) binds a maximum of two molecules of OASS (a dimer) in an interaction believed to involve docking of the C terminus from a protomer in an SAT trimer into an OASS active site. This interaction inactivates OASS catalysis and prevents further binding to the trimer; thus, the system exhibits a contact- induced inactivation of half of each biomolecule. To better understand the dynamics and energetics that underlie formation of the CSC, the interactions of OASS and SAT from Escherichia coli were studied at equilibrium and in the pre-steady state. Using an experimental strategy that initiates dissociation of the CSC at different points in the CSC-forming reaction, three stable forms of the complex were identified. Comparison of the binding behaviors of SAT and its C-terminal peptide supports a mechanism in which SAT interacts with OASS in a nonallosteric interaction involving its C terminus. This early docking event appears to fasten the proteins in close proximity and thus prepares the system to engage in a series of subsequent, energetically favorable isomerizations that inactivate OASS and produce the fully isomerized CSC.

Original languageEnglish (US)
Pages (from-to)4360-4367
Number of pages8
JournalJournal of Biological Chemistry
Volume287
Issue number6
DOIs
StatePublished - Feb 3 2012

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Cysteine Synthase
Escherichia coli
Dimers
Serine
Cysteine
Serine O-Acetyltransferase
Biosynthesis
Protein Subunits
Biomolecules
Isomerization
Catalysis
Sulfur
Metabolism
Catalytic Domain
Bacteria
Peptides
Molecules
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Three-stage assembly of the cysteine synthase complex from Escherichia coli. / Wang, Ting; Leyh, Thomas S.

In: Journal of Biological Chemistry, Vol. 287, No. 6, 03.02.2012, p. 4360-4367.

Research output: Contribution to journalArticle

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