Thiolation mediated Pegylation platform to generate functional universal red blood cells

Parimala Nacharaju, Belur N. Manjula, Seetharama A. Acharya

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


The PEGylation that adds an extension arm on protein amino groups with the conservation of their positive charge masks the A and D antigens of erythrocytes efficiently. In the present study, the efficiency of masking the antigens of RBC by PEGylation protocols that do not conserve the charge with and without adding extension arms is compared. The conjugation of PEG-5000 to RBCs through the addition of extension arms masked the D antigen more efficiently than the other protocol. A combination of PEG-5 K and PEG-20 K is needed to mask the A antigen, irrespective of the PEGylation approach. The oxygen affinity of the PEGylated RBCs increased by the extension arm facilitated PEGylation. The protocol involving the conjugation of PEG-chains without adding extension arm did not alter the oxygen affinity of RBCs. A combination of PEGylation protocols is an alternate strategy to generate universal red blood cells with good levels of oxygen affinity.

Original languageEnglish (US)
Pages (from-to)107-118
Number of pages12
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Issue number1
StatePublished - Jan 2007

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering


Dive into the research topics of 'Thiolation mediated Pegylation platform to generate functional universal red blood cells'. Together they form a unique fingerprint.

Cite this