Thermodynamic, kinetic, and electron microscopy studies of concanavalin A and Dioclea grandiflora lectin cross-linked with synthetic divalent carbohydrates

Tarun K. Dam, Stefan Oscarson, René Roy, Sanjoy K. Das, Daniel Pagé, Frank P. Macaluso, Curtis F. Brewer

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The jack bean lectin concanavalin A (ConA) and the Dioclea grandiflora lectin (DGL) are highly homologous Man/Glc-specific members of the Diocleinae subtribe. Both lectins bind, cross-link, and precipitate with carbohydrates possessing multiple terminal nonreducing Man residues. The present study investigates the binding and cross-linking interactions of ConA and DGL with a series of synthetic divalent carbohydrates that possess spacer groups with increasing flexibility and length between terminal α-mannopyranoside residues. Isothermal titration microcalorimetry was used to determine the thermodynamics of binding of the two lectins to the divalent analogs, and kinetic light scattering and electron microscopy studies were used to characterize the cross-linking interactions of the lectins with the carbohydrates. The results demonstrated that divalent analogs with flexible spacer groups between the two terminal Man residues possess higher affinities for the two lectins as compared with those with inflexible spacer groups. Furthermore, despite their high degree of homology, ConA and DGL exhibit differences in their kinetics of cross-linking and precipitation with the divalent analogs. Electron microscopy shows the loss of organized cross-linked lattices of the two lectins with analogs possessing increased distance between the terminal Man residues. The loss of lattice patterns with the analogs is distinct for each lectin. These results have important implications for the interactions of lectins with multivalent carbohydrate receptors in biological systems.

Original languageEnglish (US)
Pages (from-to)8640-8646
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number10
DOIs
StatePublished - Mar 11 2005

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Concanavalin A
Thermodynamics
Lectins
Electron microscopy
Electron Microscopy
Carbohydrates
Kinetics
Jacks
Dioclea grandiflora lectin
Biological systems
Mannose
Titration
Light scattering
Precipitates
Light

ASJC Scopus subject areas

  • Biochemistry

Cite this

Thermodynamic, kinetic, and electron microscopy studies of concanavalin A and Dioclea grandiflora lectin cross-linked with synthetic divalent carbohydrates. / Dam, Tarun K.; Oscarson, Stefan; Roy, René; Das, Sanjoy K.; Pagé, Daniel; Macaluso, Frank P.; Brewer, Curtis F.

In: Journal of Biological Chemistry, Vol. 280, No. 10, 11.03.2005, p. 8640-8646.

Research output: Contribution to journalArticle

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