The Yeast Snt2 Protein Coordinates the Transcriptional response to hydrogen peroxide-mediated oxidative stress

Lindsey A. Baker, Beatrix M. Ueberheide, Scott Dewell, Brian T. Chait, Deyou Zheng, C. David Allis

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Regulation of gene expression is a vital part of the cellular stress response, yet the full set of proteins that orchestrate this regulation remains unknown. Snt2 is a Saccharomyces cerevisiae protein whose function has not been well characterized that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Here, we confirm that Snt2, Ecm5, and Rpd3 physically associate. We then demonstrate that cells lacking Rpd3 or Snt2 are resistant to hydrogen peroxide (H2O2)-mediated oxidative stress and use chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to show that Snt2 and Ecm5 recruit Rpd3 to a small number of promoters and in response to H2O2, colocalize independently of Rpd3 to the promoters of stress response genes. By integrating ChIP-seq and expression analyses, we identify target genes that require Snt2 for proper expression after H2O2. Finally, we show that cells lacking Snt2 are also resistant to nutrient stress imparted by the TOR (target of rapamycin) pathway inhibitor rapamycin and identify a common set of genes targeted by Snt2 and Ecm5 in response to both H2O2 and rapamycin. Our results establish a function for Snt2 in regulating transcription in response to oxidative stress and suggest Snt2 may also function in multiple stress pathways.

Original languageEnglish (US)
Pages (from-to)3735-3748
Number of pages14
JournalMolecular and Cellular Biology
Volume33
Issue number19
DOIs
StatePublished - 2013

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Fungal Proteins
Sirolimus
Hydrogen Peroxide
Oxidative Stress
Genes
Saccharomyces cerevisiae Proteins
Chromatin Immunoprecipitation
Gene Expression Regulation
Food
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The Yeast Snt2 Protein Coordinates the Transcriptional response to hydrogen peroxide-mediated oxidative stress. / Baker, Lindsey A.; Ueberheide, Beatrix M.; Dewell, Scott; Chait, Brian T.; Zheng, Deyou; Allis, C. David.

In: Molecular and Cellular Biology, Vol. 33, No. 19, 2013, p. 3735-3748.

Research output: Contribution to journalArticle

Baker, Lindsey A. ; Ueberheide, Beatrix M. ; Dewell, Scott ; Chait, Brian T. ; Zheng, Deyou ; Allis, C. David. / The Yeast Snt2 Protein Coordinates the Transcriptional response to hydrogen peroxide-mediated oxidative stress. In: Molecular and Cellular Biology. 2013 ; Vol. 33, No. 19. pp. 3735-3748.
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