The ybeY protein from Escherichia coli is a metalloprotein

Chenyang Zhan, Elena V. Fedorov, Wuxian Shi, U. A. Ramagopal, R. Thirumuruhan, Babu A. Manjasetty, Steven C. Almo, Andras Fiser, Mark R. Chance, Alexander A. Fedorov

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The three-dimensional crystallographic structure of the ybeY protein from Escherichia coli (SwissProt entry P77385) is reported at 2.7 Å resolution. YbeY is a hypothetical protein that belongs to the UPF0054 family. The structure reveals that the protein binds a metal ion in a tetrahedral geometry. Three coordination sites are provided by histidine residues, while the fourth might be a water molecule that is not seen in the diffraction map because of its relatively low resolution. X-ray fluorescence analysis of the purified protein suggests that the metal is a nickel ion. The structure of ybeY and its sequence similarity to a number of predicted metal-dependent hydrolases provides a functional assignment for this protein family. The figures and tables of this paper were prepared using semi-automated tools, termed the Autopublish server, developed by the New York Structural GenomiX Research Consortium, with the goal of facilitating the rapid publication of crystallographic structures that emanate from worldwide Structural Genomics efforts, including the NIH-funded Protein Structure Initiative.

Original languageEnglish (US)
Pages (from-to)959-963
Number of pages5
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number11
DOIs
StatePublished - 2005

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Metalloproteins
Escherichia coli Proteins
Escherichia
proteins
Metals
Proteins
Ions
Protein Databases
Hydrolases
Genomics
Nickel
Histidine
histidine
Metal ions
Publications
entry
Servers
metals
Diffraction
Fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Zhan, C., Fedorov, E. V., Shi, W., Ramagopal, U. A., Thirumuruhan, R., Manjasetty, B. A., ... Fedorov, A. A. (2005). The ybeY protein from Escherichia coli is a metalloprotein. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(11), 959-963. https://doi.org/10.1107/S1744309105031131

The ybeY protein from Escherichia coli is a metalloprotein. / Zhan, Chenyang; Fedorov, Elena V.; Shi, Wuxian; Ramagopal, U. A.; Thirumuruhan, R.; Manjasetty, Babu A.; Almo, Steven C.; Fiser, Andras; Chance, Mark R.; Fedorov, Alexander A.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 11, 2005, p. 959-963.

Research output: Contribution to journalArticle

Zhan, C, Fedorov, EV, Shi, W, Ramagopal, UA, Thirumuruhan, R, Manjasetty, BA, Almo, SC, Fiser, A, Chance, MR & Fedorov, AA 2005, 'The ybeY protein from Escherichia coli is a metalloprotein', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 61, no. 11, pp. 959-963. https://doi.org/10.1107/S1744309105031131
Zhan, Chenyang ; Fedorov, Elena V. ; Shi, Wuxian ; Ramagopal, U. A. ; Thirumuruhan, R. ; Manjasetty, Babu A. ; Almo, Steven C. ; Fiser, Andras ; Chance, Mark R. ; Fedorov, Alexander A. / The ybeY protein from Escherichia coli is a metalloprotein. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2005 ; Vol. 61, No. 11. pp. 959-963.
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