The water permeability of toad urinary bladder. I. Permeability of barriers in series with the luminal membrane

S. D. Levine, M. Jacoby, A. Finkelstein

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Antidiuretic hormone (ADH) induces a large increase in the water permeability of the luminal membrane of toad urinary bladder. Measured values of the diffusional water permeability coefficient, P(d)(w), are spuriously low, however, because of barriers within the tissue, in series with the luminal membrane, that impede diffusion. We have now determined the water permeability coefficient of these series barriers in fully stretched bladders and find it to be ~ 6.3 x 10-4 cm/s. This is equivalent to an unstirred aqueous layer of ~ 400 μm. On the other hand, the permeability coefficient of the bladder to a lipophilic molecule, hexanol, is ~ 9.0 x 10-4 cm/s. This is equivalent to an unstirred aqueous layer of only 100 μm. The much smaller hindrance to hexanol diffusion than to water diffusion by the series barriers implies a lipophilic component to the barriers. We suggest that membrane-enclosed organelles may be so tightly packed within the cytoplasm of granular epithelial cells that they offer a substantial impediment to diffusion of water through the cell. Alternatively, the lipophilic component of the barrier could be the plasma membranes of the basal cells, which cover most of the basement membrane and thereby may restrict water transport to the narrow spaces between basal and granular cells.

Original languageEnglish (US)
Pages (from-to)529-541
Number of pages13
JournalJournal of General Physiology
Volume83
Issue number4
StatePublished - 1984

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Anura
Permeability
Urinary Bladder
Membranes
Water
Hexanols
Vasopressins
Basement Membrane
Organelles
Cytoplasm
Epithelial Cells
Cell Membrane

ASJC Scopus subject areas

  • Physiology

Cite this

The water permeability of toad urinary bladder. I. Permeability of barriers in series with the luminal membrane. / Levine, S. D.; Jacoby, M.; Finkelstein, A.

In: Journal of General Physiology, Vol. 83, No. 4, 1984, p. 529-541.

Research output: Contribution to journalArticle

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