The usher N terminus is the initial targeting site for chaperone-subunit complexes and participates in subsequent pilus biogenesis events

Tony W. Ng, Leyla Akman, Mary Osisami, David G. Thanassi

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Pilus biogenesis on the surface of uropathogenic Escherichia coli requires the chaperone/usher pathway, a terminal branch of the general secretory pathway. In this pathway, periplasmic chaperone-subunit complexes target an outer membrane (OM) usher for subunit assembly into pili and secretion to the cell surface. The molecular mechanisms of protein secretion across the OM are not well understood. Mutagenesis of the P pilus usher PapC and the type 1 pilus usher FimD was undertaken to elucidate the initial stages of pilus biogenesis at the OM. Deletion of residues 2 to 11 of the mature PapC N terminus abolished the targeting of the usher by chaperone-subunit complexes and rendered PapC nonfunctional for pilus biogenesis. Similarly, an intact FimD N terminus was required for chaperone-subunit binding and pilus biogenesis. Analysis of PapC-FimD chimeras and N-terminal fragments of PapC localized the chaperone-subunit targeting domain to the first 124 residues of PapC. Single alanine substitution mutations were made in this domain that blocked pilus biogenesis but did not affect targeting of chaperone-subunit complexes. Thus, the usher N terminus does not function simply as a static binding site for chaperone-subunit complexes but also participates in subsequent pilus assembly events.

Original languageEnglish (US)
Pages (from-to)5321-5331
Number of pages11
JournalJournal of Bacteriology
Volume186
Issue number16
DOIs
StatePublished - Aug 2004
Externally publishedYes

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Membranes
Uropathogenic Escherichia coli
Secretory Pathway
Mutagenesis
Alanine
Binding Sites
Mutation
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The usher N terminus is the initial targeting site for chaperone-subunit complexes and participates in subsequent pilus biogenesis events. / Ng, Tony W.; Akman, Leyla; Osisami, Mary; Thanassi, David G.

In: Journal of Bacteriology, Vol. 186, No. 16, 08.2004, p. 5321-5331.

Research output: Contribution to journalArticle

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