The use of fluorescent proteins for intravital imaging of cancer cell invasion

James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David R. Entenberg, Jeffrey Wyckoff, John S. Condeelis, Jeffrey E. Segall

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

The analysis of cancer cell behavior in the primary tumor in living animals provides an opportunity to explore the process of invasion and intravasation in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages15-30
Number of pages16
Volume872
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume872
ISSN (Print)10643745

Fingerprint

Neoplasms
Proteins
Tumor Cell Line
Dissection
Breast
Breast Neoplasms
Skin
Injections
Intravital Microscopy

Keywords

  • Intravital imaging
  • Live animal imaging
  • Multiphoton imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Hulit, J., Kedrin, D., Gligorijevic, B., Entenberg, D. R., Wyckoff, J., Condeelis, J. S., & Segall, J. E. (2012). The use of fluorescent proteins for intravital imaging of cancer cell invasion. In Methods in Molecular Biology (Vol. 872, pp. 15-30). (Methods in Molecular Biology; Vol. 872). https://doi.org/10.1007/978-1-61779-797-2_2

The use of fluorescent proteins for intravital imaging of cancer cell invasion. / Hulit, James; Kedrin, Dmitriy; Gligorijevic, Bojana; Entenberg, David R.; Wyckoff, Jeffrey; Condeelis, John S.; Segall, Jeffrey E.

Methods in Molecular Biology. Vol. 872 2012. p. 15-30 (Methods in Molecular Biology; Vol. 872).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hulit, J, Kedrin, D, Gligorijevic, B, Entenberg, DR, Wyckoff, J, Condeelis, JS & Segall, JE 2012, The use of fluorescent proteins for intravital imaging of cancer cell invasion. in Methods in Molecular Biology. vol. 872, Methods in Molecular Biology, vol. 872, pp. 15-30. https://doi.org/10.1007/978-1-61779-797-2_2
Hulit J, Kedrin D, Gligorijevic B, Entenberg DR, Wyckoff J, Condeelis JS et al. The use of fluorescent proteins for intravital imaging of cancer cell invasion. In Methods in Molecular Biology. Vol. 872. 2012. p. 15-30. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-797-2_2
Hulit, James ; Kedrin, Dmitriy ; Gligorijevic, Bojana ; Entenberg, David R. ; Wyckoff, Jeffrey ; Condeelis, John S. ; Segall, Jeffrey E. / The use of fluorescent proteins for intravital imaging of cancer cell invasion. Methods in Molecular Biology. Vol. 872 2012. pp. 15-30 (Methods in Molecular Biology).
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