The use of fluorescent proteins for intravital imaging of cancer cell invasion

James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg, Jeffrey Wyckoff, John Condeelis, Jeffrey E. Segall

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

The analysis of cancer cell behavior in the primary tumor in living animals provides an opportunity to explore the process of invasion and intravasation in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window.

Original languageEnglish (US)
Title of host publicationIn Vivo Cellular Imaging Using Fluorescent Proteins
Subtitle of host publicationMethods and Protocols
EditorsRobert M. Hoffman
Pages15-30
Number of pages16
DOIs
StatePublished - Dec 1 2012

Publication series

NameMethods in Molecular Biology
Volume872
ISSN (Print)1064-3745

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Keywords

  • Intravital imaging
  • Live animal imaging
  • Multiphoton imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hulit, J., Kedrin, D., Gligorijevic, B., Entenberg, D., Wyckoff, J., Condeelis, J., & Segall, J. E. (2012). The use of fluorescent proteins for intravital imaging of cancer cell invasion. In R. M. Hoffman (Ed.), In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols (pp. 15-30). (Methods in Molecular Biology; Vol. 872). https://doi.org/10.1007/978-1-61779-797-2_2