The use of DREADDs (Designer receptors exclusively activated by designer receptors) in transgenic mouse behavioral models

Daniela Cassataro, Lucas L. Sjulson

Research output: Contribution to journalArticle

Abstract

Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) are increasingly used to manipulate activity in specific neuronal populations in the brains of awake, behaving mice. Here we review the pros and cons of DREADDs relative to other genetically encoded neuromodulation technologies and describe in detail methods for using DREADDs with transgenic mouse behavioral models. This approach can not only provide insight into the role of specific neural circuits in behavior but also identify potential neuromodulation targets for the treatment of neuropsychiatric disorders.

Original languageEnglish (US)
Pages (from-to)95-108
Number of pages14
JournalNeuromethods
Volume108
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Designer Drugs
Transgenic Mice
Brain
Technology
Networks (circuits)
Population

Keywords

  • Addiction
  • CNO
  • DREADDs
  • Mouse behavior
  • Reverse pharmacogenetics
  • Transgenic mice

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

The use of DREADDs (Designer receptors exclusively activated by designer receptors) in transgenic mouse behavioral models. / Cassataro, Daniela; Sjulson, Lucas L.

In: Neuromethods, Vol. 108, 01.01.2015, p. 95-108.

Research output: Contribution to journalArticle

@article{0a53fe6a4394489886094e4d3a8e97f8,
title = "The use of DREADDs (Designer receptors exclusively activated by designer receptors) in transgenic mouse behavioral models",
abstract = "Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) are increasingly used to manipulate activity in specific neuronal populations in the brains of awake, behaving mice. Here we review the pros and cons of DREADDs relative to other genetically encoded neuromodulation technologies and describe in detail methods for using DREADDs with transgenic mouse behavioral models. This approach can not only provide insight into the role of specific neural circuits in behavior but also identify potential neuromodulation targets for the treatment of neuropsychiatric disorders.",
keywords = "Addiction, CNO, DREADDs, Mouse behavior, Reverse pharmacogenetics, Transgenic mice",
author = "Daniela Cassataro and Sjulson, {Lucas L.}",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-2944-3_6",
language = "English (US)",
volume = "108",
pages = "95--108",
journal = "Neuromethods",
issn = "0893-2336",
publisher = "Humana Press",

}

TY - JOUR

T1 - The use of DREADDs (Designer receptors exclusively activated by designer receptors) in transgenic mouse behavioral models

AU - Cassataro, Daniela

AU - Sjulson, Lucas L.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) are increasingly used to manipulate activity in specific neuronal populations in the brains of awake, behaving mice. Here we review the pros and cons of DREADDs relative to other genetically encoded neuromodulation technologies and describe in detail methods for using DREADDs with transgenic mouse behavioral models. This approach can not only provide insight into the role of specific neural circuits in behavior but also identify potential neuromodulation targets for the treatment of neuropsychiatric disorders.

AB - Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) are increasingly used to manipulate activity in specific neuronal populations in the brains of awake, behaving mice. Here we review the pros and cons of DREADDs relative to other genetically encoded neuromodulation technologies and describe in detail methods for using DREADDs with transgenic mouse behavioral models. This approach can not only provide insight into the role of specific neural circuits in behavior but also identify potential neuromodulation targets for the treatment of neuropsychiatric disorders.

KW - Addiction

KW - CNO

KW - DREADDs

KW - Mouse behavior

KW - Reverse pharmacogenetics

KW - Transgenic mice

UR - http://www.scopus.com/inward/record.url?scp=84941084654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941084654&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2944-3_6

DO - 10.1007/978-1-4939-2944-3_6

M3 - Article

AN - SCOPUS:84941084654

VL - 108

SP - 95

EP - 108

JO - Neuromethods

JF - Neuromethods

SN - 0893-2336

ER -