The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants

Gadi Spira, Polly D. Gregor, Matthew D. Scharff

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.

Original languageEnglish (US)
Pages (from-to)263-268
Number of pages6
JournalJournal of Immunological Methods
Volume165
Issue number2
DOIs
StatePublished - Oct 15 1993

Fingerprint

Luminescence
Immunoglobulins
Enzyme-Linked Immunosorbent Assay
Collodion
Cell Count
Membranes

Keywords

  • Automatic counting
  • Chemiluminescence
  • ELISA spot
  • Isotype switching

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants. / Spira, Gadi; Gregor, Polly D.; Scharff, Matthew D.

In: Journal of Immunological Methods, Vol. 165, No. 2, 15.10.1993, p. 263-268.

Research output: Contribution to journalArticle

@article{b8e33c562f3b4a05a224a671b75b6ffc,
title = "The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants",
abstract = "The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.",
keywords = "Automatic counting, Chemiluminescence, ELISA spot, Isotype switching",
author = "Gadi Spira and Gregor, {Polly D.} and Scharff, {Matthew D.}",
year = "1993",
month = "10",
day = "15",
doi = "10.1016/0022-1759(93)90352-8",
language = "English (US)",
volume = "165",
pages = "263--268",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants

AU - Spira, Gadi

AU - Gregor, Polly D.

AU - Scharff, Matthew D.

PY - 1993/10/15

Y1 - 1993/10/15

N2 - The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.

AB - The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.

KW - Automatic counting

KW - Chemiluminescence

KW - ELISA spot

KW - Isotype switching

UR - http://www.scopus.com/inward/record.url?scp=0027515580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027515580&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(93)90352-8

DO - 10.1016/0022-1759(93)90352-8

M3 - Article

VL - 165

SP - 263

EP - 268

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 2

ER -