The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants

Gadi Spira; Polly D. Gregor; Matthew D. Scharff

doi:10.1016/0022-1759(93)90352-8

The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants

Gadi Spira, Polly D. Gregor, Matthew D. Scharff

Cell Biology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.

Original language	English (US)
Pages (from-to)	263-268
Number of pages	6
Journal	Journal of Immunological Methods
Volume	165
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(93)90352-8
State	Published - Oct 15 1993

Keywords

Automatic counting
Chemiluminescence
ELISA spot
Isotype switching

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(93)90352-8

Cite this

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title = "The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants",

abstract = "The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.",

keywords = "Automatic counting, Chemiluminescence, ELISA spot, Isotype switching",

author = "Gadi Spira and Gregor, {Polly D.} and Scharff, {Matthew D.}",

note = "Funding Information: We would like to thank PeiYing Yang for her technical assistance and Dr. Melia Paizi for helping in the preparation of the manuscript. This study was carried out in the hybridoma facility and in the Computer Center of the Cancer Center and supported by grants from the United States/Israel Binational Research Fund (89-00310) and in part by the National Institute of Health (CA39838, PO1CA1330). Dr. Scharff is supported in part by the Harry Eagle Chair for Cancer Research from the National Women's Division of the Albert Einstein College of Medicine.",

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doi = "10.1016/0022-1759(93)90352-8",

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AU - Gregor, Polly D.

AU - Scharff, Matthew D.

N1 - Funding Information: We would like to thank PeiYing Yang for her technical assistance and Dr. Melia Paizi for helping in the preparation of the manuscript. This study was carried out in the hybridoma facility and in the Computer Center of the Cancer Center and supported by grants from the United States/Israel Binational Research Fund (89-00310) and in part by the National Institute of Health (CA39838, PO1CA1330). Dr. Scharff is supported in part by the Harry Eagle Chair for Cancer Research from the National Women's Division of the Albert Einstein College of Medicine.

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N2 - The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.

AB - The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.

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KW - ELISA spot

KW - Isotype switching

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The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants

Abstract

Keywords

ASJC Scopus subject areas

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