The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants

Gadi Spira, Polly D. Gregor, Matthew D. Scharff

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.

Original languageEnglish (US)
Pages (from-to)263-268
Number of pages6
JournalJournal of Immunological Methods
Volume165
Issue number2
DOIs
StatePublished - Oct 15 1993

Keywords

  • Automatic counting
  • Chemiluminescence
  • ELISA spot
  • Isotype switching

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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