The unfolded protein response in the protozoan parasite Toxoplasma gondii features translational and transcriptional control

Bradley R. Joyce, Zoi Tampaki, Kami Kim, Ronald C. Wek, William J. Sullivan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The unfolded protein response (UPR) is an important regulatory network that responds to perturbations in protein homeostasis in the endoplasmic reticulum (ER). In mammalian cells, the UPR features translational and transcriptional mechanisms of gene expression aimed at restoring proteostatic control. A central feature of the UPR is phosphorylation of the subunit of eukaryotic initiation factor-2 (eIF2) by PERK (EIF2AK3/PEK), which reduces the influx of nascent proteins into the ER by lowering global protein synthesis, coincident with preferential translation of key transcription activators of genes that function to expand the processing capacity of this secretory organelle. Upon ER stress, the apicomplexan parasite Toxoplasma gondii is known to induce phosphorylation of Toxoplasma eIF2 and lower translation initiation. To characterize the nature of the ensuing UPR in this parasite, we carried out microarray analyses to measure the changes in the transcriptome and in translational control during ER stress. We determined that a collection of transcripts linked with the secretory process are induced in response to ER stress, supporting the idea that a transcriptional induction phase of the UPR occurs in Toxoplasma. Furthermore, we determined that about 500 gene transcripts showed enhanced association with translating ribosomes during ER stress. Many of these target genes are suggested to be involved in gene expression, including JmjC5, which continues to be actively translated during ER stress. This study indicates that Toxoplasma triggers a UPR during ER stress that features both translational and transcriptional regulatory mechanisms, which is likely to be important for parasite invasion and development.

Original languageEnglish (US)
Pages (from-to)979-989
Number of pages11
JournalEukaryotic Cell
Volume12
Issue number7
DOIs
StatePublished - Jul 2013

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Unfolded Protein Response
Endoplasmic Reticulum Stress
Toxoplasma
Parasites
Eukaryotic Initiation Factor-2
Endoplasmic Reticulum
Phosphorylation
Activator Appliances
Genes
Gene Expression
Proteins
Secretory Pathway
Microarray Analysis
Ribosomes
Transcriptome
Organelles
Homeostasis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

The unfolded protein response in the protozoan parasite Toxoplasma gondii features translational and transcriptional control. / Joyce, Bradley R.; Tampaki, Zoi; Kim, Kami; Wek, Ronald C.; Sullivan, William J.

In: Eukaryotic Cell, Vol. 12, No. 7, 07.2013, p. 979-989.

Research output: Contribution to journalArticle

Joyce, Bradley R. ; Tampaki, Zoi ; Kim, Kami ; Wek, Ronald C. ; Sullivan, William J. / The unfolded protein response in the protozoan parasite Toxoplasma gondii features translational and transcriptional control. In: Eukaryotic Cell. 2013 ; Vol. 12, No. 7. pp. 979-989.
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