The tyrosine-containing cytoplasmic tail of CD1b is essential for its efficient presentation of bacterial lipid antigens

Robin M. Jackman, Steffen Stenger, Agnes Lee, D. Branch Moody, Rick A. Rogers, Kayvan R. Niazi, Masahiko Sugita, Robert L. Modlin, Peter J. Peters, Steven A. Porcelli

Research output: Contribution to journalArticle

134 Scopus citations

Abstract

CD1b is an antigen-presenting molecule that mediates recognition of bacterial lipid and glycolipid antigens by specific T cells. We demonstrate that the nine-amino acid cytoplasmic tail of CD1b contains all of the signals required for its normal endosomal targeting, and that the single cytoplasmic tyrosine is a critical component of the targeting motif. Mutant forms of CD1b lacking the endosomal targeting motif are expressed at high levels on the cell surface but are unable to efficiently present lipid antigens acquired either exogenously or from live intracellular organisms. These results define the functional role of the CD1b targeting motif in a physiologic setting and demonstrate its importance in delivery of this antigen-presenting molecule to appropriate intracellular compartments.

Original languageEnglish (US)
Pages (from-to)341-351
Number of pages11
JournalImmunity
Volume8
Issue number3
DOIs
StatePublished - Mar 1998
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Jackman, R. M., Stenger, S., Lee, A., Moody, D. B., Rogers, R. A., Niazi, K. R., Sugita, M., Modlin, R. L., Peters, P. J., & Porcelli, S. A. (1998). The tyrosine-containing cytoplasmic tail of CD1b is essential for its efficient presentation of bacterial lipid antigens. Immunity, 8(3), 341-351. https://doi.org/10.1016/S1074-7613(00)80539-7