The trk proto-oncogene rescues NGF responsiveness in mutant NGF-nonresponsive PC12 cell lines

David M. Loeb, Johnna Maragos, Dionisio Martin-Zanca, Moses V. Chao, Luis F. Parada, Lloyd A. Greene

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

The trk tyrosine kinase proto-oncogene product gp140prototrk binds nerve growth factor (NGF) and is rapidly and selectively activated by this neurotrophic factor. To determine whether gp140prototrk is involved in transducing a functional NGF signal, PC12 cell mutants (PC12nnr) deficient in high affinity NGF binding and unresponsive to NGF were used. Northern analysis revealed that these mutant cells have greatly reduced levels of trk expression. PC12nnr cultures were transiently transfected with expression vectors encoding the full-length rat trk cDNA and assessed for responsiveness to NGF. Expression of exogenous trk rescued the capacity for NGF-promoted neurite outgrowth, cellular hypertrophy, and serum-free survival by these cells. These results indicate that gp140prototrk is necessary for functional NGF signal transduction.

Original languageEnglish (US)
Pages (from-to)961-966
Number of pages6
JournalCell
Volume66
Issue number5
DOIs
StatePublished - Sep 6 1991
Externally publishedYes

Fingerprint

Proto-Oncogenes
PC12 Cells
Nerve Growth Factor
Cells
Cell Line
Signal transduction
Oncogene Proteins
Nerve Growth Factors
Protein-Tyrosine Kinases
Hypertrophy
Rats
Signal Transduction
Cell Survival
Complementary DNA
Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Loeb, D. M., Maragos, J., Martin-Zanca, D., Chao, M. V., Parada, L. F., & Greene, L. A. (1991). The trk proto-oncogene rescues NGF responsiveness in mutant NGF-nonresponsive PC12 cell lines. Cell, 66(5), 961-966. https://doi.org/10.1016/0092-8674(91)90441-Z

The trk proto-oncogene rescues NGF responsiveness in mutant NGF-nonresponsive PC12 cell lines. / Loeb, David M.; Maragos, Johnna; Martin-Zanca, Dionisio; Chao, Moses V.; Parada, Luis F.; Greene, Lloyd A.

In: Cell, Vol. 66, No. 5, 06.09.1991, p. 961-966.

Research output: Contribution to journalArticle

Loeb, DM, Maragos, J, Martin-Zanca, D, Chao, MV, Parada, LF & Greene, LA 1991, 'The trk proto-oncogene rescues NGF responsiveness in mutant NGF-nonresponsive PC12 cell lines', Cell, vol. 66, no. 5, pp. 961-966. https://doi.org/10.1016/0092-8674(91)90441-Z
Loeb, David M. ; Maragos, Johnna ; Martin-Zanca, Dionisio ; Chao, Moses V. ; Parada, Luis F. ; Greene, Lloyd A. / The trk proto-oncogene rescues NGF responsiveness in mutant NGF-nonresponsive PC12 cell lines. In: Cell. 1991 ; Vol. 66, No. 5. pp. 961-966.
@article{595ccbe2249e4c568e4da99add4696b7,
title = "The trk proto-oncogene rescues NGF responsiveness in mutant NGF-nonresponsive PC12 cell lines",
abstract = "The trk tyrosine kinase proto-oncogene product gp140prototrk binds nerve growth factor (NGF) and is rapidly and selectively activated by this neurotrophic factor. To determine whether gp140prototrk is involved in transducing a functional NGF signal, PC12 cell mutants (PC12nnr) deficient in high affinity NGF binding and unresponsive to NGF were used. Northern analysis revealed that these mutant cells have greatly reduced levels of trk expression. PC12nnr cultures were transiently transfected with expression vectors encoding the full-length rat trk cDNA and assessed for responsiveness to NGF. Expression of exogenous trk rescued the capacity for NGF-promoted neurite outgrowth, cellular hypertrophy, and serum-free survival by these cells. These results indicate that gp140prototrk is necessary for functional NGF signal transduction.",
author = "Loeb, {David M.} and Johnna Maragos and Dionisio Martin-Zanca and Chao, {Moses V.} and Parada, {Luis F.} and Greene, {Lloyd A.}",
year = "1991",
month = "9",
day = "6",
doi = "10.1016/0092-8674(91)90441-Z",
language = "English (US)",
volume = "66",
pages = "961--966",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - The trk proto-oncogene rescues NGF responsiveness in mutant NGF-nonresponsive PC12 cell lines

AU - Loeb, David M.

AU - Maragos, Johnna

AU - Martin-Zanca, Dionisio

AU - Chao, Moses V.

AU - Parada, Luis F.

AU - Greene, Lloyd A.

PY - 1991/9/6

Y1 - 1991/9/6

N2 - The trk tyrosine kinase proto-oncogene product gp140prototrk binds nerve growth factor (NGF) and is rapidly and selectively activated by this neurotrophic factor. To determine whether gp140prototrk is involved in transducing a functional NGF signal, PC12 cell mutants (PC12nnr) deficient in high affinity NGF binding and unresponsive to NGF were used. Northern analysis revealed that these mutant cells have greatly reduced levels of trk expression. PC12nnr cultures were transiently transfected with expression vectors encoding the full-length rat trk cDNA and assessed for responsiveness to NGF. Expression of exogenous trk rescued the capacity for NGF-promoted neurite outgrowth, cellular hypertrophy, and serum-free survival by these cells. These results indicate that gp140prototrk is necessary for functional NGF signal transduction.

AB - The trk tyrosine kinase proto-oncogene product gp140prototrk binds nerve growth factor (NGF) and is rapidly and selectively activated by this neurotrophic factor. To determine whether gp140prototrk is involved in transducing a functional NGF signal, PC12 cell mutants (PC12nnr) deficient in high affinity NGF binding and unresponsive to NGF were used. Northern analysis revealed that these mutant cells have greatly reduced levels of trk expression. PC12nnr cultures were transiently transfected with expression vectors encoding the full-length rat trk cDNA and assessed for responsiveness to NGF. Expression of exogenous trk rescued the capacity for NGF-promoted neurite outgrowth, cellular hypertrophy, and serum-free survival by these cells. These results indicate that gp140prototrk is necessary for functional NGF signal transduction.

UR - http://www.scopus.com/inward/record.url?scp=0026091934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026091934&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(91)90441-Z

DO - 10.1016/0092-8674(91)90441-Z

M3 - Article

VL - 66

SP - 961

EP - 966

JO - Cell

JF - Cell

SN - 0092-8674

IS - 5

ER -