The telomere-associated MAL3 locus of Saccharomyces is a tandem array of repeated genes

C. A. Michels, E. Read, K. Nat, Maureen J. Charron

Research output: Contribution to journalArticle

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Abstract

Saccharomyces strains capable of fermenting maltose contain any one of five telomere-associated MAL loci. Each MAL locus is a complex of three genes encoding the three functions required to ferment maltose: maltose permease (GENE 1), maltase (GENE 2) and MAL trans-activator (GENE 3). All five loci have been cloned and all are highly sequence homologous over at least a 9.0 kbp region containing these GENEs (Charron et al., Genetics 122, 307-331, 1989). Our initial studies of strains carrying the MAL3 locus indicated the presence of linked, repeated MAL-homologous sequences (Michels and Needleman, Mol. Gen. Genet. 191, 225-230, 1983). Here we report our analysis of the centromere-proximal MAL3-linked sequences and show that the complete MAL3 locus spans approximately 40 kbp and consists of tandemly arrayed, partial repeats of the three GENE sequences described above. In addition, the structure of the MAL3 locus is compared to that of three partially functional alleles of MAL3. These alleles were shown to contain only MAL31 and MAL32 and their structure suggests that they resulted from MAL3 deletions removing the sequences centromere-proximal to MAL31. The amplification and rearrangement of the telomere-linked MAL3 sequences are discussed in the context of studies on other telemere-associated sequences from yeast and other species.

Original languageEnglish (US)
Pages (from-to)655-665
Number of pages11
JournalYeast
Volume8
Issue number8
StatePublished - 1992

Fingerprint

Maltose
Saccharomyces
Centromere
telomeres
Telomere
Sequence Homology
Genes
Alleles
Viverridae
loci
Gene encoding
alpha-Glucosidases
Trans-Activators
Sequence Deletion
maltose
Yeast
Amplification
genes
Yeasts
centromeres

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

The telomere-associated MAL3 locus of Saccharomyces is a tandem array of repeated genes. / Michels, C. A.; Read, E.; Nat, K.; Charron, Maureen J.

In: Yeast, Vol. 8, No. 8, 1992, p. 655-665.

Research output: Contribution to journalArticle

Michels, C. A. ; Read, E. ; Nat, K. ; Charron, Maureen J. / The telomere-associated MAL3 locus of Saccharomyces is a tandem array of repeated genes. In: Yeast. 1992 ; Vol. 8, No. 8. pp. 655-665.
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