The TATA binding protein in the sea urchin embryo is maternally derived

Lisa Edelmann, Lianxing Zheng, Zeng Feng Wang, William Marzluff, Gary M. Wessel, Geoffrey J. Childs

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The cDNA encoding the TATA binding protein was isolated from 8- to 16- cell and morula-stage embryonic libraries of two distantly related species of sea urchin, Strongylocentrotus purpuratus and Lytechinus variegatus, respectively. The two proteins are 96% identical over both the N- and C- terminal domains, suggesting a conservation of transcriptional processes between the two species. The prevalence of SpTBP transcripts at several developmental time points was determined using the tracer excess titration method, and the corresponding number of TBP protein molecules was determined by quantitative Western blot analysis. Our results indicate that the amount of TBP mRNA and protein per embryo remains relatively constant throughout development. An initial large pool of TBP protein (>109) molecules in the egg becomes diluted as a consequence of cell division and decreases to about 2 x 106 molecules per cell by the gastrula stage. We found by in situ RNA hybridization that the oocyte contains a large amount of TBP mRNA which is depleted late in oogenesis so that the eggs and early embryos have extremely low levels of TBP mRNA. We conclude that the oocyte manufactures nearly all of the TBP protein necessary for embryogenesis.

Original languageEnglish (US)
Pages (from-to)293-304
Number of pages12
JournalDevelopmental Biology
Volume204
Issue number1
DOIs
StatePublished - Dec 1 1998

Fingerprint

TATA-Box Binding Protein
Sea Urchins
Embryonic Structures
Proteins
Messenger RNA
Oocytes
Lytechinus
Strongylocentrotus purpuratus
Morula
Gastrula
Oogenesis
Cell Division
Eggs
Libraries
Embryonic Development
In Situ Hybridization
Ovum
tributyl phosphate
Complementary DNA
Western Blotting

Keywords

  • Maternal supply
  • Sea urchin
  • TATA binding protein

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The TATA binding protein in the sea urchin embryo is maternally derived. / Edelmann, Lisa; Zheng, Lianxing; Wang, Zeng Feng; Marzluff, William; Wessel, Gary M.; Childs, Geoffrey J.

In: Developmental Biology, Vol. 204, No. 1, 01.12.1998, p. 293-304.

Research output: Contribution to journalArticle

Edelmann, L, Zheng, L, Wang, ZF, Marzluff, W, Wessel, GM & Childs, GJ 1998, 'The TATA binding protein in the sea urchin embryo is maternally derived', Developmental Biology, vol. 204, no. 1, pp. 293-304. https://doi.org/10.1006/dbio.1998.9052
Edelmann, Lisa ; Zheng, Lianxing ; Wang, Zeng Feng ; Marzluff, William ; Wessel, Gary M. ; Childs, Geoffrey J. / The TATA binding protein in the sea urchin embryo is maternally derived. In: Developmental Biology. 1998 ; Vol. 204, No. 1. pp. 293-304.
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