The SLE-key test serological signature: New insights into the course of lupus

Chaim Putterman, David S. Pisetsky, Michelle Petri, Roberto Caricchio, Alan H.B. Wu, Ignacio Sanz, Jim C. Oates, Steve Wallace, Rachel Sorek, Robert Gerwien, Pennina Safer, Keren Jakobi-Brook, Irun R. Cohen

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Objective. We previously described the multiplex autoantibody SLE-key Rule-Out test, which detects a signature of autoantibody reactivity that distinguishes healthy subjects from SLE patients with 94% sensitivity, 75% specificity and 93% negative predictive value; thus, an individual manifesting a positive Rule-Out test score is unlikely to have SLE (e.g. lupus is excluded). The objective of this current study was to evaluate the stability of the lupus-associated signature over time. Methods. We used banked serum samples from healthy subjects (n = 51) and lupus patients (n = 50 individual samples and n = 181 paired samples, for a total of n = 412 serum samples). The samples were drawn at different times after diagnosis to analyse the impact on the SLE-key Rule-Out test of time elapsed since diagnosis and any changes in disease activity (as reflected by the SLEDAI score). Results. The SLE signature remains stable for the first 10 years after diagnosis; in this time frame, <10% of patients manifested a positive Rule-Out score and the SLE-key Rule-Out score was independent of the underlying disease activity as reflected by the SLEDAI score. After 510 years, 30% of lupus subjects scored as SLE Ruled-Out; the proportion of patients manifesting this status was even greater in the subset of individuals with a SLEDAI score of 0. Conclusion. These findings raise the possibility that a significant number of SLE patients manifest a change in their serological signature over time, and that such a signature change may signify an evolution in the immunological features of their disease relevant to patient management.

Original languageEnglish (US)
Pages (from-to)1632-1640
Number of pages9
JournalRheumatology (United Kingdom)
Volume57
Issue number9
DOIs
StatePublished - Jan 1 2018

Keywords

  • Autoantibodies
  • Diagnosis
  • Microarray
  • Multivariate classifier
  • Natural history
  • SLE-key
  • Systemic lupus erythematosus
  • iCHIP

ASJC Scopus subject areas

  • Rheumatology
  • Pharmacology (medical)

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    Putterman, C., Pisetsky, D. S., Petri, M., Caricchio, R., Wu, A. H. B., Sanz, I., Oates, J. C., Wallace, S., Sorek, R., Gerwien, R., Safer, P., Jakobi-Brook, K., & Cohen, I. R. (2018). The SLE-key test serological signature: New insights into the course of lupus. Rheumatology (United Kingdom), 57(9), 1632-1640. https://doi.org/10.1093/rheumatology/key149