The Scabrous protein can act as an extracellular antagonist of Notch signaling in the Drosophila wing

E. Chiang Lee, Sung Yun Yu, Nicholas E. Baker

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Notch (N) is a receptor for signals that inhibit neural precursor specification [1-6]. As N and its ligand Delta (Dl) are expressed homogeneously, other molecules may be differentially expressed or active to permit neural precursor cells to arise intermingled with non-neural cells [7,8]. During Drosophila wing development, the glycosyltransferase encoded by the gene fringe (fng) promotes N signaling in response to Dl, but inhibits N signaling in response to Serrate (Ser), which encodes a ligand that is structurally similar to Dl. Dorsal expression of Fng protein localizes N signaling to the dorsoventral (DV) wing margin [9-11]. The secreted protein Scabrous (Sca) is a candidate for modulation of N in neural cells. Mutations at the scabrous (sca) locus alter the locations where precursor cells form in the peripheral nervous system [12,13]. Unlike fringe, sca mutations act cell non-autonomously [12]. Here, we report that targeted misexpression of Sca during wing development inhibited N signaling, blocking expression of all N target genes. Sca reduced N activation in response to Dl more than in response to Ser. Ligand-independent signaling by overexpression of N protein, or by expression of activated truncated N molecules, was not inhibited by Sca. Our results indicate that Sca can act on N to reduce its availability for paracrine and autocrine interactions with Dl and Ser, and can act as an antagonist of N signaling.

Original languageEnglish (US)
Pages (from-to)931-934
Number of pages4
JournalCurrent Biology
Volume10
Issue number15
DOIs
StatePublished - Aug 1 2000

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Drosophila
antagonists
Ligands
Proteins
proteins
cells
mutation
glycosyltransferases
peripheral nervous system
Genes
Glycosyltransferases
Mutation
Peripheral Nervous System
Molecules
genes
protein synthesis
neurons
Neurology
receptors
loci

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

The Scabrous protein can act as an extracellular antagonist of Notch signaling in the Drosophila wing. / Lee, E. Chiang; Yu, Sung Yun; Baker, Nicholas E.

In: Current Biology, Vol. 10, No. 15, 01.08.2000, p. 931-934.

Research output: Contribution to journalArticle

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